ABCC8 p.Gly20Ser
| Predicted by SNAP2: | A: D (53%), C: D (59%), D: N (61%), E: D (66%), F: D (63%), H: N (53%), I: D (53%), K: D (71%), L: D (63%), M: D (63%), N: N (82%), P: D (66%), Q: D (59%), R: D (66%), S: N (78%), T: N (57%), V: D (59%), W: D (66%), Y: D (53%), |
| Predicted by PROVEAN: | A: N, C: D, D: N, E: D, F: D, H: D, I: D, K: D, L: D, M: D, N: N, P: D, Q: D, R: D, S: N, T: D, V: D, W: D, Y: D, |
[switch to compact view]
Comments [show]
None has been submitted yet.
[hide] Carbapenems as inhibitors of OXA-13, a novel, inte... Microbiology. 1998 Apr;144 ( Pt 4):1021-31. Mugnier P, Podglajen I, Goldstein FW, Collatz E
Carbapenems as inhibitors of OXA-13, a novel, integron-encoded beta-lactamase in Pseudomonas aeruginosa.
Microbiology. 1998 Apr;144 ( Pt 4):1021-31., [PMID:9579076]
Abstract [show]
A clinical Pseudomonas aeruginosa strain, PAe391, was found to be resistant to a number of antibiotics including ticarcillin, piperacillin, cefsulodin and amikacin, and a disk diffusion assay showed evidence of pronounced synergy between imipenem and various beta-lactam antibiotics. Cloning and nucleotide sequence analysis revealed the dicistronic arrangement of an aac(6')-Ib variant and a novel blaOXA-type gene between the intI and qacE delta 1 genes typical of integrons, in PAe391, this integron was apparently chromosome-borne. The beta-lactamase, named OXA-13, displayed nine amino acid changes with respect to OXA-10:I in position 10 of OXA-10 to T (I10T), G20S, D55N, N73S, T107S, Y174F, E229G, S245N and E259A, OXA-13 (pIapp = 8.0) showed poor catalytic activity against penicillins as well as cephalosporins, but was efficient in hydrolysing some penicillinase-resistant beta-lactams, such as cefotaxime and aztreonam. It was efficiently inhibited by imipenem (KIapp = 11 nM), and formed a stable complex. While the KIapp value of meropenem was similar (16 nM), the corresponding complex was less stable.
Comments [show]
None has been submitted yet.
No. Sentence Comment
5 The /?- lactamase, named OXA-13, displayed nine amino acid changes with respect to OXA-10: Iin position 10 of OXA-10 to T (HOT), G20S, D55N, N73S, T107S, Y174F, E229G, S245N and E259A.
X
ABCC8 p.Gly20Ser 9579076:5:129
status: NEW