ABCC7 p.Leu859Ala
| Predicted by SNAP2: | A: N (66%), C: N (82%), D: D (66%), E: D (59%), F: N (93%), G: D (59%), H: N (61%), I: N (93%), K: D (53%), M: N (93%), N: N (53%), P: D (63%), Q: N (57%), R: N (53%), S: N (57%), T: N (72%), V: N (87%), W: N (72%), Y: N (87%), |
| Predicted by PROVEAN: | A: N, C: N, D: D, E: D, F: N, G: D, H: D, I: N, K: D, M: N, N: D, P: D, Q: D, R: D, S: D, T: N, V: N, W: D, Y: N, |
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[hide] Identification of a region of strong discriminatio... Am J Physiol Lung Cell Mol Physiol. 2001 Oct;281(4):L852-67. McCarty NA, Zhang ZR
Identification of a region of strong discrimination in the pore of CFTR.
Am J Physiol Lung Cell Mol Physiol. 2001 Oct;281(4):L852-67., [PMID:11557589]
Abstract [show]
The variety of methods used to identify the structural determinants of anion selectivity in the cystic fibrosis transmembrane conductance regulator Cl(-) channel has made it difficult to assemble the data into a coherent framework that describes the three-dimensional structure of the pore. Here, we compare the relative importance of sites previously studied and identify new sites that contribute strongly to anion selectivity. We studied Cl(-) and substitute anions in oocytes expressing wild-type cystic fibrosis transmembrane conductance regulator or 12-pore-domain mutants to determine relative permeability and relative conductance for 9 monovalent anions and 1 divalent anion. The data indicate that the region of strong discrimination resides between T338 and S341 in transmembrane 6, where mutations affected selectivity between Cl(-) and both large and small anions. Mutations further toward the extracellular end of the pore only strongly affected selectivity between Cl(-) and larger anions. Only mutations at S341 affected selectivity between monovalent and divalent anions. The data are consistent with a narrowing of the pore between the extracellular end and a constriction near the middle of the pore.
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No. Sentence Comment
261 Discrimination between anions could result from differences in energy barrier heights (reflected in Px/PCl) or from differences in well depths (reflected in L859A Gx/GCl).
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ABCC7 p.Leu859Ala 11557589:261:157
status: NEW