ABCC1 p.Met1524Gly
| Predicted by SNAP2: | A: D (59%), C: N (53%), D: D (75%), E: D (75%), F: D (59%), G: D (71%), H: D (66%), I: N (57%), K: D (75%), L: N (78%), N: D (66%), P: D (75%), Q: D (63%), R: D (71%), S: D (63%), T: D (59%), V: N (53%), W: D (71%), Y: D (63%), |
| Predicted by PROVEAN: | A: D, C: D, D: D, E: D, F: D, G: D, H: D, I: D, K: D, L: N, N: D, P: D, Q: D, R: D, S: D, T: D, V: D, W: D, Y: D, |
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[hide] Identification and characterization of functionall... J Biol Chem. 2004 Dec 17;279(51):53571-83. Epub 2004 Sep 30. Westlake CJ, Payen L, Gao M, Cole SP, Deeley RG
Identification and characterization of functionally important elements in the multidrug resistance protein 1 COOH-terminal region.
J Biol Chem. 2004 Dec 17;279(51):53571-83. Epub 2004 Sep 30., 2004-12-17 [PMID:15459206]
Abstract [show]
The ATP binding cassette (ABC) transporter, multidrug resistance protein 1 (MRP1/ABCC1), transports a broad spectrum of conjugated and unconjugated compounds, including natural product chemotherapeutic agents. In this study, we have investigated the importance of the COOH-terminal region of MRP1 for transport activity and basolateral plasma membrane trafficking. The COOH-terminal regions of some ABCC proteins have been implicated in protein trafficking, but the function of this region of MRP1 has not been defined. In contrast to results obtained with other ABCC proteins, we found that the COOH-proximal 30 amino acids of MRP1 can be removed without affecting trafficking to basolateral membranes. However, the truncated protein is inactive. Furthermore, removal of as few as 4 COOH-terminal amino acids profoundly decreases transport activity. Although amino acid sequence conservation of the COOH-terminal regions of ABC proteins is low, secondary structure predictions indicate that they consist of a broadly conserved helix-sheet-sheet-helix-helix structure. Consistent with a conservation of secondary and tertiary structure, MRP1 hybrids containing the COOH-terminal regions of either the homologous MRP2 or the distantly related P-glycoprotein were fully active and trafficked normally. Using mutated proteins, we have identified structural elements containing five conserved hydrophobic amino acids that are required for activity. We show that these are important for binding and hydrolysis of ATP by nucleotide binding domain 2. Based on crystal structures of several ABC proteins, we suggest that the conserved amino acids may stabilize a helical bundle formed by the COOH-terminal three helices and may contribute to interactions between the COOH-terminal region and the protein's two nucleotide binding domains.
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No. Sentence Comment
247 In contrast to Ala substitution, the M1524G mutation decreased LTC4 transport by ϳ35%.
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ABCC1 p.Met1524Gly 15459206:247:37
status: NEW248 Furthermore, although a single mutation of the adjacent Ala1524 to Gly did not affect activity, the double mutation M1524G/A1525G reduced LTC4 transport by ϳ75% (Table I).
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ABCC1 p.Met1524Gly 15459206:248:116
status: NEW256 Related structure COOH-terminal mutation Expression (relative to WT) LTC4 uptake (percentage of WT) Km Vmax -fold % M pmol mg-1 min-1 Helix C3 L1488A 1.2 48 Ϯ 2 I1491A 1.2 59 Ϯ 2 M1492A 1.4 89 Ϯ 3 Sheet C2 V1499A 1.0 94 Ϯ 6 L1500A 1.1 22 Ϯ 1 V1497A/I1498A/V1499A/L1500A 1.0 3.6 Ϯ 0.8 D1501A 1.0 90 Ϯ 5 Sheet C1 G1503A 0.8 100 Ϯ 4 I1505A 0.5 90 Ϯ 10 E1507A 0.8 108 Ϯ 6 G1509A 0.8 113 Ϯ 9 Helix C2 P1511A 1.0 82 Ϯ 6 L1514A/L1515A 1.2 18 Ϯ 2 623 84 L1514V/L1515V 1.0 75 Ϯ 1 L1514I/L1515I 1.0 94 Ϯ 2 Q1516A 1.2 94 Ϯ 3 R1518A 0.8 103 Ϯ 10 G1519A 1.1 100 Ϯ 10 L1520A 1.3 110 Ϯ 3 Helix C1 F1521A 0.8 38 Ϯ 1 F1521Y 1.0 105 Ϯ 2 F1521G 0.9 40 Ϯ 1 F1521Q 1.0 30 Ϯ 1 F1521E 1.0 22 Ϯ 1 Y1522A 1.2 90 Ϯ 5 S1523A 1.1 103 Ϯ 6 M1524A 1.0 130 Ϯ 2 M1524G 1.0 65 Ϯ 3 A1525G 1.0 100 Ϯ 4 M1524G/A1525G 1.0 21 Ϯ 3 733 68 K1526A 2.5 85 Ϯ 2 D1527A/G1529A 3.0 123 Ϯ 4 tation impaired nucleotide binding and trapping at NBD2 to an extent comparable with that resulting from removal of the COOH-terminal 4 amino acids (not shown).
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ABCC1 p.Met1524Gly 15459206:256:899
status: NEWX
ABCC1 p.Met1524Gly 15459206:256:948
status: NEW279 This suggestion is further supported by the effect of introducing destabilizing Gly mutations into this helix, as exemplified by the double M1524G/A1525G mutation, which had the same effect on activity as deletion of the terminal 4 or 9 amino acids.
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ABCC1 p.Met1524Gly 15459206:279:140
status: NEW