ABCC1 p.Leu1515Ile
| Predicted by SNAP2: | A: N (57%), C: N (72%), D: D (66%), E: D (59%), F: N (82%), G: D (59%), H: D (53%), I: N (97%), K: D (53%), M: N (97%), N: D (53%), P: D (63%), Q: N (53%), R: D (59%), S: N (53%), T: N (61%), V: N (82%), W: N (57%), Y: N (66%), |
| Predicted by PROVEAN: | A: D, C: D, D: D, E: D, F: D, G: D, H: D, I: N, K: D, M: N, N: D, P: D, Q: D, R: D, S: D, T: D, V: N, W: D, Y: D, |
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[hide] Identification and characterization of functionall... J Biol Chem. 2004 Dec 17;279(51):53571-83. Epub 2004 Sep 30. Westlake CJ, Payen L, Gao M, Cole SP, Deeley RG
Identification and characterization of functionally important elements in the multidrug resistance protein 1 COOH-terminal region.
J Biol Chem. 2004 Dec 17;279(51):53571-83. Epub 2004 Sep 30., 2004-12-17 [PMID:15459206]
Abstract [show]
The ATP binding cassette (ABC) transporter, multidrug resistance protein 1 (MRP1/ABCC1), transports a broad spectrum of conjugated and unconjugated compounds, including natural product chemotherapeutic agents. In this study, we have investigated the importance of the COOH-terminal region of MRP1 for transport activity and basolateral plasma membrane trafficking. The COOH-terminal regions of some ABCC proteins have been implicated in protein trafficking, but the function of this region of MRP1 has not been defined. In contrast to results obtained with other ABCC proteins, we found that the COOH-proximal 30 amino acids of MRP1 can be removed without affecting trafficking to basolateral membranes. However, the truncated protein is inactive. Furthermore, removal of as few as 4 COOH-terminal amino acids profoundly decreases transport activity. Although amino acid sequence conservation of the COOH-terminal regions of ABC proteins is low, secondary structure predictions indicate that they consist of a broadly conserved helix-sheet-sheet-helix-helix structure. Consistent with a conservation of secondary and tertiary structure, MRP1 hybrids containing the COOH-terminal regions of either the homologous MRP2 or the distantly related P-glycoprotein were fully active and trafficked normally. Using mutated proteins, we have identified structural elements containing five conserved hydrophobic amino acids that are required for activity. We show that these are important for binding and hydrolysis of ATP by nucleotide binding domain 2. Based on crystal structures of several ABC proteins, we suggest that the conserved amino acids may stabilize a helical bundle formed by the COOH-terminal three helices and may contribute to interactions between the COOH-terminal region and the protein's two nucleotide binding domains.
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No. Sentence Comment
241 Consequently, we created two additional double mutations, L1514V/L1515V and L1514I/L1515I.
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ABCC1 p.Leu1515Ile 15459206:241:83
status: NEW256 Related structure COOH-terminal mutation Expression (relative to WT) LTC4 uptake (percentage of WT) Km Vmax -fold % M pmol mg-1 min-1 Helix C3 L1488A 1.2 48 Ϯ 2 I1491A 1.2 59 Ϯ 2 M1492A 1.4 89 Ϯ 3 Sheet C2 V1499A 1.0 94 Ϯ 6 L1500A 1.1 22 Ϯ 1 V1497A/I1498A/V1499A/L1500A 1.0 3.6 Ϯ 0.8 D1501A 1.0 90 Ϯ 5 Sheet C1 G1503A 0.8 100 Ϯ 4 I1505A 0.5 90 Ϯ 10 E1507A 0.8 108 Ϯ 6 G1509A 0.8 113 Ϯ 9 Helix C2 P1511A 1.0 82 Ϯ 6 L1514A/L1515A 1.2 18 Ϯ 2 623 84 L1514V/L1515V 1.0 75 Ϯ 1 L1514I/L1515I 1.0 94 Ϯ 2 Q1516A 1.2 94 Ϯ 3 R1518A 0.8 103 Ϯ 10 G1519A 1.1 100 Ϯ 10 L1520A 1.3 110 Ϯ 3 Helix C1 F1521A 0.8 38 Ϯ 1 F1521Y 1.0 105 Ϯ 2 F1521G 0.9 40 Ϯ 1 F1521Q 1.0 30 Ϯ 1 F1521E 1.0 22 Ϯ 1 Y1522A 1.2 90 Ϯ 5 S1523A 1.1 103 Ϯ 6 M1524A 1.0 130 Ϯ 2 M1524G 1.0 65 Ϯ 3 A1525G 1.0 100 Ϯ 4 M1524G/A1525G 1.0 21 Ϯ 3 733 68 K1526A 2.5 85 Ϯ 2 D1527A/G1529A 3.0 123 Ϯ 4 tation impaired nucleotide binding and trapping at NBD2 to an extent comparable with that resulting from removal of the COOH-terminal 4 amino acids (not shown).
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ABCC1 p.Leu1515Ile 15459206:256:570
status: NEW302 Consistent with the presence of Leu-Val and Leu-Ile, rather than di-Leu, in some ABC proteins, substitution of Leu1514 and Leu1515 with Ile or Val rather than Ala had minimal effect on LTC4 transport activity.
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ABCC1 p.Leu1515Ile 15459206:302:123
status: NEW