ABCC7 p.Lys1041Cys
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PMID: 25944907
[PubMed]
El Hiani Y et al: "Functional Architecture of the Cytoplasmic Entrance to the Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel Pore."
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Sentence
Comment
109
Application of MTSES (200 òe;M) following channel activation with PKA and ATP never caused an increase in macroscopic current amplitude but decreased current amplitude in K190C, R248C, R251C, R303C, K370C, K946C, R975C, K1041C, and R1048C (Figs. 2 and 3).
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ABCC7 p.Lys1041Cys 25944907:109:224
status: NEW110 The effect of MTSET on these MTSES-sensitive mutants was to increase (K190C and R303C), decrease (R248C, K946C, K1041C, and R1048C), or have no effect (R251C, K370C, and R975C) on macroscopic current amplitude (Figs. 2 and 3).
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ABCC7 p.Lys1041Cys 25944907:110:112
status: NEW120 Inhibitory effects of MTS reagents on K1041C were diminished but not completely abolished following PPi treatment (Fig. 5).
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ABCC7 p.Lys1041Cys 25944907:120:38
status: NEW132 Error bars represent the means afe; S.E. from three to five patches. Cytoplasmic Entrance to the CFTR Channel Pore JUNE 19, 2015ߦVOLUME 290ߦNUMBER 25 JOURNAL OF BIOLOGICAL CHEMISTRY 15859 rent amplitude in each of K190C, R248C, R303C, K370C, K1041C, and R1048C.
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ABCC7 p.Lys1041Cys 25944907:132:258
status: NEW141 Cytoplasmic Entrance to the CFTR Channel Pore 15860 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 290ߦNUMBER 25ߦJUNE 19, 2015 at SEMMELWEIS UNIV OF MEDICINE on December 4, amplitudes in unmodified channels was Cys-less b; K946C b03; R975C b0e; K370C b0e; R251C b0e; K1041C b03; R248C b0e; R1048C b0e; R303C b0e; K190C (Fig. 7A).
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ABCC7 p.Lys1041Cys 25944907:141:287
status: NEW142 Among channels showing MTSES-sensitive current amplitudes, the order following modification was K1041C b0e; R1048C b0e; K370C b0e; R248C b0e; R303C b0e; K190C (Fig. 6, B and C, and 7B).
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ABCC7 p.Lys1041Cys 25944907:142:96
status: NEW155 Single channel recording indicated directly that for each of K190C, R248C, R303C, K370C, K1041C, and R1048C Clafa; conductance was reduced relative to Cys-less (Figs. 6 and 7A), Clafa; conductance was further reduced by modification by negatively charged MTSES (Figs. 6 and 7B), and Clafa; conductance was restored to near wild-type (Cys-less) values by modification by positively charged MTSET (Figs. 6 and 7C).
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ABCC7 p.Lys1041Cys 25944907:155:89
status: NEW167 Only in K1041C was there a discrepancy between MTS effects on single channel current amplitude and on macroscopic current amplitude following PPi treatment.
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ABCC7 p.Lys1041Cys 25944907:167:8
status: NEW180 Error bars represent the means afe; S.E. from 3-10 patches. Cytoplasmic Entrance to the CFTR Channel Pore 15862 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 290ߦNUMBER 25ߦJUNE 19, 2015 at SEMMELWEIS UNIV OF MEDICINE on December , when compared with K1041C and R1048C (Figs. 6C and 7B).
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ABCC7 p.Lys1041Cys 25944907:180:259
status: NEW182 Modification by MTSES had a lesser effect on unitary current amplitude in K1041C (b03;59% of Cys-less) and R1048C (b03;38% of Cys-less) (Fig. 6, B and C, and 7B) perhaps because these positive charges are located further from the major Clafa; permeation pathway.
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ABCC7 p.Lys1041Cys 25944907:182:74
status: NEW