ABCC7 p.Arg251Cys
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PMID: 25944907
[PubMed]
El Hiani Y et al: "Functional Architecture of the Cytoplasmic Entrance to the Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel Pore."
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Sentence
Comment
109
Application of MTSES (200 òe;M) following channel activation with PKA and ATP never caused an increase in macroscopic current amplitude but decreased current amplitude in K190C, R248C, R251C, R303C, K370C, K946C, R975C, K1041C, and R1048C (Figs. 2 and 3).
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ABCC7 p.Arg251Cys 25944907:109:189
status: NEW110 The effect of MTSET on these MTSES-sensitive mutants was to increase (K190C and R303C), decrease (R248C, K946C, K1041C, and R1048C), or have no effect (R251C, K370C, and R975C) on macroscopic current amplitude (Figs. 2 and 3).
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ABCC7 p.Arg251Cys 25944907:110:152
status: NEW119 The effects of MTS reagents on R251C were similarly lost following PPi treatment, suggesting that this mutant also had little or no effect on Clafa; conductance (Fig. 5).
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ABCC7 p.Arg251Cys 25944907:119:31
status: NEW127 Current amplitude in R251C was slightly increased by MTSET but not significantly altered by MTSES (Figs. 6 and 7).
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ABCC7 p.Arg251Cys 25944907:127:21
status: NEW141 Cytoplasmic Entrance to the CFTR Channel Pore 15860 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 290ߦNUMBER 25ߦJUNE 19, 2015 at SEMMELWEIS UNIV OF MEDICINE on December 4, amplitudes in unmodified channels was Cys-less b; K946C b03; R975C b0e; K370C b0e; R251C b0e; K1041C b03; R248C b0e; R1048C b0e; R303C b0e; K190C (Fig. 7A).
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ABCC7 p.Arg251Cys 25944907:141:273
status: NEW164 Consistent with this, the functional effect of MTS reagents on R251C, K946C, and R975C were abolished in channels that had been treated with PPi to maintain the channels in the open state (Fig. 5).
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ABCC7 p.Arg251Cys 25944907:164:63
status: NEW