ABCMdb

ABCC7 p.Lys370Cys

None has been submitted yet.

Publications

PMID: 25944907 [PubMed] El Hiani Y et al: "Functional Architecture of the Cytoplasmic Entrance to the Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel Pore."

No. Sentence Comment

103 A-D, examples of modification of four different mutants (A, R303C, used in this study as a positive control; B, K190C; C, K370C; D, R1048C) by MTSES (left panels) or MTSET (right panels). Login to comment
109 Application of MTSES (200 òe;M) following channel activation with PKA and ATP never caused an increase in macroscopic current amplitude but decreased current amplitude in K190C, R248C, R251C, R303C, K370C, K946C, R975C, K1041C, and R1048C (Figs. 2 and 3). Login to comment
110 The effect of MTSET on these MTSES-sensitive mutants was to increase (K190C and R303C), decrease (R248C, K946C, K1041C, and R1048C), or have no effect (R251C, K370C, and R975C) on macroscopic current amplitude (Figs. 2 and 3). Login to comment
121 In contrast, the inhibitory effects of MTSES on K190C, R248C, K370C, and R1048C were preserved following PPi treatment (Figs. 4 and 5), suggesting that modification with this negatively charged reagent caused a major inhibition of Clafa; conductance in open channels in each of these mutants. Login to comment
122 Interestingly, in each of these four mutants, MTSET caused a significant increase in macroscopic current amplitude when applied after PPi treatment (Figs. 4 and 5) even though MTSET applied in the absence of PPi treatment decreased macroscopic current amplitude in R248C, K370C, and R1048C (Figs. 4 and 5). Login to comment
123 This implies that modification with positively charged MTSET caused an increase in Clafa; conductance in each of K190C, R248C, R303C, K370C, and R1048C, an effect that may potentially be masked by inhibitory effects on channel open probability in normally gating channels. Login to comment
132 Error bars represent the means afe; S.E. from three to five patches. Cytoplasmic Entrance to the CFTR Channel Pore JUNE 19, 2015ߦVOLUME 290ߦNUMBER 25 JOURNAL OF BIOLOGICAL CHEMISTRY 15859 rent amplitude in each of K190C, R248C, R303C, K370C, K1041C, and R1048C. Login to comment
141 Cytoplasmic Entrance to the CFTR Channel Pore 15860 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 290ߦNUMBER 25ߦJUNE 19, 2015 at SEMMELWEIS UNIV OF MEDICINE on December 4, amplitudes in unmodified channels was Cys-less b; K946C b03; R975C b0e; K370C b0e; R251C b0e; K1041C b03; R248C b0e; R1048C b0e; R303C b0e; K190C (Fig. 7A). Login to comment
142 Among channels showing MTSES-sensitive current amplitudes, the order following modification was K1041C b0e; R1048C b0e; K370C b0e; R248C b0e; R303C b0e; K190C (Fig. 6, B and C, and 7B). Login to comment
155 Single channel recording indicated directly that for each of K190C, R248C, R303C, K370C, K1041C, and R1048C Clafa; conductance was reduced relative to Cys-less (Figs. 6 and 7A), Clafa; conductance was further reduced by modification by negatively charged MTSES (Figs. 6 and 7B), and Clafa; conductance was restored to near wild-type (Cys-less) values by modification by positively charged MTSET (Figs. 6 and 7C). Login to comment
166 In contrast, the effects of MTS modification of PPi-treated channels in K190C, R248C, R303C, K370C, and R1048C (Fig. 5) closely mirrored the charge-dependent effects of MTS modification on single channel current amplitude (Figs. 6 and 7). Login to comment
173 We believe that the functional importance of Lys-190, Arg-248, Arg-303, and Lys-370 is likely greater than that of Lys-1041 and Arg-1048 because of the greater reduction in single channel current amplitude following introduction of a negative charge by MTSES modification in K190C, R248C, R303C, and K370C FIGURE 6. Login to comment
181 Single channel current amplitude was very low in MTSES- modified K190C, R248C, R303C, and K370C channels (b0d;25% of Cys-less current amplitude; Figs. 6, B and C, and 7B), suggesting that most permeating Clafa; ions must pass close to these residues. Login to comment