ABCC7 p.Arg975Ala
[switch to full view]Comments [show]
None has been submitted yet.
PMID: 17582383
[PubMed]
Melin P et al: "CFTR inhibition by glibenclamide requires a positive charge in cytoplasmic loop three."
No.
Sentence
Comment
4
Mutations of the neighbouring R975 (R975A, R975S, R975Q) did not affect electrophysiological and pharmacological properties of CFTR.
X
ABCC7 p.Arg975Ala 17582383:4:36
status: NEW114 After expression in HEK-293 cells, the whole-cell chloride currents recorded with charge neutralizing mutants CFTR-R975A, R975Q and R975S (Fig. 5) showed similar electrophysiological properties as CFTR-wt: linear activation with Fsk, inhibition by glibenclamide and by CFTRinh-172.
X
ABCC7 p.Arg975Ala 17582383:114:115
status: NEW151 Original currents recorded on HEK-293 transfected with EGFP-CFTR-R975A with Fsk 10 bc; M (A), Fsk+Glib 100 bc;M (B), and Fsk+CFTRinh-172 10 bc;M (C).
X
ABCC7 p.Arg975Ala 17582383:151:65
status: NEW152 (D) Representative time course of current densities from the charge neutralizing mutant EGFP-CFTR-R975A in presence of Fsk 10 bc;M, Fsk+Glib 100 bc;M, Fsk+CFTRinh-172 10 bc;M.
X
ABCC7 p.Arg975Ala 17582383:152:98
status: NEW153 (E) Current-voltage relationships normalized by cell capacitance recorded from R975A (n=11), R975S (n=7) and R975Q (n=4) EGFP-CFTR channels in presence of agonists.
X
ABCC7 p.Arg975Ala 17582383:153:79
status: NEW