ABCA3 p.Asn945Gln
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PMID: 24142515
[PubMed]
Beers MF et al: "Disruption of N-linked glycosylation promotes proteasomal degradation of the human ATP-binding cassette transporter ABCA3."
No.
Sentence
Comment
47
The primers [primer nucleotide sequence is obtained from the National Center for Biotechnology (NCBI) of human ABCA3, data accession number NM_001089] generated for these mutant constructs are as follows: for N53Q: forward, 5=-tcggaaaatgtgccccaggccaccatctacccg-3=, reverse, 5=-cgggtagatggtggcctggggcacattttccga-3=; for N124Q: forward, 5=-ctacattaggtacgaccagtgctcgtccagcgtgc-3=, reverse, 5=-gcacgctggacgag- cactggtcgtacctaatgtag-3=; for N140Q, forward, 5=-tcgagcaccccttccagca- cagcaaggagcc-3=, reverse, 5=-ggctccttgctgtgctggaaggggtgctcga-3=, and for N945Q: forward, 5=-ccctcctggccatccagtactcctcggagct-3=, reverse, 5=- agctccgaggagtactggatggccaggaggg-3=.
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ABCA3 p.Asn945Gln 24142515:47:549
status: NEW247 A: representative confocal images of A549 cells 24 h following plasmid introduction of EGFP-tagged to either WT or a mutant (N945Q) ABCA3 isoform showing predominant localization of both isoforms within CD63-positive LROs.
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ABCA3 p.Asn945Gln 24142515:247:125
status: NEW248 Bar, 5 òe;m. B: representative anti-GFP immunoblots (from 2 separate experiments) of whole cell lysates of A549 cells NT or transfected with either WT or N945Q mutant EGFP-tagged ABCA3 cDNAs showing similar band electrophoretic mobility and protein expression levels.
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ABCA3 p.Asn945Gln 24142515:248:158
status: NEW