ABCG2 p.Met131Ala
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PMID: 26294421
[PubMed]
Haider AJ et al: "Identification of residues in ABCG2 affecting protein trafficking and drug transport, using co-evolutionary analysis of ABCG sequences."
No.
Sentence
Comment
109
We made individual substitutions of each amino acid to alanine: M131A, S195A, K453A, K473A, P485A, M549A, W564A and I573A.
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ABCG2 p.Met131Ala 26294421:109:64
status: NEW128 Transfection reagent only (PEI) served as negative control, WT His12-ABCG2 and His12-M131A serve as positive controls.
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ABCG2 p.Met131Ala 26294421:128:85
status: NEW135 WT and a representative other mutant (His12-M131A) are fully glycosylated at both 24 and 48 h post transfection.
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ABCG2 p.Met131Ala 26294421:135:44
status: NEW153 Firstly, there was a group of five mutants which showed FTC-inhibited MX export comparable to WT protein (e.g. M131A, S195A, K453A, K473A, W564A; M131A data shown in Figure 3C).
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ABCG2 p.Met131Ala 26294421:153:111
status: NEWX
ABCG2 p.Met131Ala 26294421:153:146
status: NEW165 Data are representative of at least four independent transfections and reflect negative control data (empty vector, A), positive control data (WT ABCG2 expression, B), a mutant with normal MX transport function (M131A, C) and a mutant with abrogated function but normal surface expression (P485A, D).
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ABCG2 p.Met131Ala 26294421:165:212
status: NEW