ABCMdb

ABCG2 p.Ser552Met

None has been submitted yet.

Publications

PMID: 18094074 [PubMed] Velamakanni S et al: "A functional steroid-binding element in an ATP-binding cassette multidrug transporter."

No. Sentence Comment

28 Mutations in the ABCG2R482G gene were introduced using the forward primer 5b18;-TTT TTT CAC GTC TGT TGG TCA ATC TCA C-3b18; and the reverse primer 5b18;-ATT GAC CAA CAG ACG TGA AAA AAT CAT C-3b18; for G553R, the forward primer 5b18;-GAT GAT TTT TAT GGG TCT GTT GGT CAA TCT CAC-3b18; and reverse primer 5b18;-CCA ACA GAC CCA TAA AAA TCA TCA TAA ACA C-3b18; for S552M, and the forward primer 5b18;-GGT CTG GGG GTC AAT GGC ACA ACC ATT GCA TCT TGG-3b18; and reverse primer 5b18;- ATG GTT GTG CCA TTG ACC CCC AGA CCT GAA AAA ATC-3b18; for L555A L558A. Login to comment
83 The Ser552 to methionine substitution (as observed in hERॷ) did not alter protein expression (Fig. 2A and SM mutant) or the interaction of ABCG2R482G with PG (Fig. 2D) but decreased the apparent Kd value for ED 6-fold (from 4.7 afe; 0.3 òe;M for ABCG2R482G to 0.8 afe; 0.2 òe;M for SM mutant), whereas the Bmax was unaffected (106.6 afe; 7.4 nmol/mg of protein for ABCG2R482G versus 104.2 afe; 5.5 Fig. 2. Login to comment