ABCA1 p.Leu439Ala
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PMID: 26173179
[PubMed]
Ito A et al: "LXRs link metabolism to inflammation through Abca1-dependent regulation of membrane composition and TLR signaling."
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Sentence
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46
(C) Immortalized MEFs from Lxrb1;-/-Lxrb2; -/- mice reconstituted with wild-type human LXRb1;, L439A/E441A mutant or control mock were pretreated with the LXR agonist GW3965 (1 bc;M) overnight, followed by stimulation with LPS (10 ng/ml) for 4 hr. Gene expression was analyzed by real-time PCR.
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ABCA1 p.Leu439Ala 26173179:46:104
status: NEW54 Furthermore, an LXR mutant defective in its ability to recruit co-activators, L439A/E441A (Tzukerman et al., 1994; Bastie et al., 2000), was unable to induce Abca1 or to repress inflammatory genes in both MEFs and iBMDM (Figure 1C, Figure 2B, Figure 2-figure supplement 1A), strongly suggesting that gene activation and inflammatory repression are mechanistically linked.
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ABCA1 p.Leu439Ala 26173179:54:78
status: NEW80 (B, C) Immortalized bone marrow-derived macrophages from Lxrb1;-/-Lxrb2;-/- mice reconstituted with wild-type human LXRb1;, K328R/K434R (KK) mutant, L439A/E441A mutant, or mock control were pretreated with GW3965 (1 bc;M) overnight, followed by stimulation with LPS (10 ng/ml) for 4 hr. Gene expression was analyzed by real-time PCR (B) and Agilent microarrays (C).
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ABCA1 p.Leu439Ala 26173179:80:158
status: NEW184 We found that an LXR L439A/E441A mutant that lacks co-activator recruitment capacity was unable to repress inflammation.
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ABCA1 p.Leu439Ala 26173179:184:21
status: NEW