ABCC6 p.Asn15Asp
[switch to full view]Comments [show]
None has been submitted yet.
PMID: 24840500
[PubMed]
Xue P et al: "Regulation of ABCC6 trafficking and stability by a conserved C-terminal PDZ-like sequence."
No.
Sentence
Comment
115
To further confirm these species were the result of changes in glycosylation, the single glycosylation site was mutated (N15D) and expressed.
X
ABCC6 p.Asn15Asp 24840500:115:121
status: NEW117 Migration of the single N15D protein band was consistent with the fully deglycosylated protein, band A, in the wildtype ABCC6 expressing cells. Deletion of the six C-terminal amino acids (D6-COOH), which includes the complete PDZ ligands found in other ABCC family members, showed dramatic changes in both protein biosynthesis and protein trafficking as measured by western blotting (Figure 1C, D).
X
ABCC6 p.Asn15Asp 24840500:117:24
status: NEW135 No observable changes in immunofluorescence were seen between the wildtype and N15D glycosylation mutant (data not shown).
X
ABCC6 p.Asn15Asp 24840500:135:79
status: NEW195 The N15D substitution blocks N-linked glycosylation and is a reference for the unglycosylated wildtype and D6-COOH proteins.
X
ABCC6 p.Asn15Asp 24840500:195:4
status: NEW