ABCB4 p.Phe770Cys
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PMID: 26507655
[PubMed]
Loo TW et al: "Mapping the Binding Site of the Inhibitor Tariquidar That Stabilizes the First Transmembrane Domain of P-glycoprotein."
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Sentence
Comment
63
Disulfide Cross-linking Analysis-Cys-less P-gp mutants containing pairs of cross-linkable cysteines in the TM segments (A80C(TM1)/R741C(TM7), I299C(TM5)/F770C (TM8), and T333C(TM6)/L975C(TM12)), intracellular loops (ICLs) (L175C(ICL1)/N820C(ICL3), A259C(ICL2)/ W803C(ICL3), and A266C(ICL2)/F1086C(NBD2)), or the NBDs (C431(NBD1)/L1176C(NBD2), L531C(NBD1)/ C1074(NBD2), and P517C(NBD1)/I1050C(NBD2)) were transiently expressed in HEK 293 cells at reduced temperature (30 &#b0;C) to promote maturation.
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ABCB4 p.Phe770Cys 26507655:63:153
status: NEW65 Membranes were prepared and samples were incubated at 0 &#b0;C (A80C/R741C, A259C/W803C, I299C/F770C, and A266C/ F1086C) or 20 &#b0;C (L175C/N820C, C431/L1176C, and L521C/C1074) for 10 min in the presence or absence of 0.5 Mapping the P-glycoprotein Tariquidar-binding Site 29390 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 290ߦNUMBER 49ߦDECEMBER 4, 2015 at SEMMELWEIS UNIV OF MEDICINE on December 11, mM copper phenanthroline (oxidant to promote disulfide bond formation) in the presence or absence of 0.25 òe;M (for TM segment cysteine mutants) or 1 òe;M tariquidar (for the ICL and NBD cysteine mutants).
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ABCB4 p.Phe770Cys 26507655:65:95
status: NEW