ABCB4 p.Ala250Leu
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PMID: 25987565
[PubMed]
Loo TW et al: "The Transmission Interfaces Contribute Asymmetrically to the Assembly and Activity of Human P-glycoprotein."
No.
Sentence
Comment
152
Asymmetry of the P-gp Drug Pump Transmission Interfaces JULY 3, 2015ߦVOLUME 290ߦNUMBER 27 JOURNAL OF BIOLOGICAL CHEMISTRY 16957 at SEMMELWEIS UNIV OF MEDICINE on December 4, twenty-eight mutations in ICL2 (A250L, G251V, V253S, A254L, E256A, L258S, I261S, V264S, F267A, G268V, G269V, L274S, R276A, Y277A) inhibited maturation of P-gp (b0d;15% mature P-gp) while four other ICL2 mutations (A2650L, R262A, T263A, and I265S) partially reduced maturation of P-gp (about 55-60% mature P-gp).
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ABCB4 p.Ala250Leu 25987565:152:219
status: NEW160 Rescue of a representative misprocessed mutant by tariquidar is shown on Fig. 3A. No detectable mature P-gp was observed when mutant A250L (ICL2) was expressed in the absence of drug substrate.
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ABCB4 p.Ala250Leu 25987565:160:133
status: NEW161 Expression in the presence of tariquidar however, promoted maturation of the A250L mutant to yield mature 170 kDa P-gp as the major product.
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ABCB4 p.Ala250Leu 25987565:161:77
status: NEW188 A, representative immunoblot of SDS extracts of whole cells transfected with wild-type P-gp (WT) or mutant A250L.
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ABCB4 p.Ala250Leu 25987565:188:107
status: NEW