ABCB1 p.Phe904Cys
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PMID: 25987565
[PubMed]
Loo TW et al: "The Transmission Interfaces Contribute Asymmetrically to the Assembly and Activity of Human P-glycoprotein."
No.
Sentence
Comment
92
Disulfide Cross-linking Analysis-Cys-less P-gp or the ICL1/ ICL4 double cysteine mutants I160C/F904C or F163C/R905C were transiently expressed in HEK 293 cells at reduced temperature (30 &#b0;C) to promote maturation.
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ABCB1 p.Phe904Cys 25987565:92:95
status: NEW223 Mutants I160C/F904C and F163/R905C (Fig. 5, A and B) were selected to test the effects of IH1/IH4 cross-linking as the mutants yielded mature P-gp that showed efficient cross-linking in the presence of oxidant (copper phenanthroline) (Fig. 5C).
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ABCB1 p.Phe904Cys 25987565:223:14
status: NEW226 No cross-linked product was observed when the I160C, F904C, F163C, or R905C mutants were treated with oxidant (data not shown).
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ABCB1 p.Phe904Cys 25987565:226:53
status: NEW227 To test for the effects of cross-linking on activity, isolated Cys-less P-gp and mutants I160C/F904C and F163/R905C were treated without or with copper phenanthroline for 10 min a 20 &#b0;C.
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ABCB1 p.Phe904Cys 25987565:227:95
status: NEW239 Mutants I160C/ F904C and F163C/R905C exhibited over 70% of the activity of the Cys-less parent (Fig. 5D).
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ABCB1 p.Phe904Cys 25987565:239:15
status: NEW240 Cross-linking of mutants I160C/ F904C and F163C/R905C with oxidant however, inhibited more than 90% of the activity.
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ABCB1 p.Phe904Cys 25987565:240:32
status: NEW