ABCB1 p.Phe163Cys
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PMID: 25987565
[PubMed]
Loo TW et al: "The Transmission Interfaces Contribute Asymmetrically to the Assembly and Activity of Human P-glycoprotein."
No.
Sentence
Comment
92
Disulfide Cross-linking Analysis-Cys-less P-gp or the ICL1/ ICL4 double cysteine mutants I160C/F904C or F163C/R905C were transiently expressed in HEK 293 cells at reduced temperature (30 &#b0;C) to promote maturation.
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ABCB1 p.Phe163Cys 25987565:92:104
status: NEW226 No cross-linked product was observed when the I160C, F904C, F163C, or R905C mutants were treated with oxidant (data not shown).
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ABCB1 p.Phe163Cys 25987565:226:60
status: NEW239 Mutants I160C/ F904C and F163C/R905C exhibited over 70% of the activity of the Cys-less parent (Fig. 5D).
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ABCB1 p.Phe163Cys 25987565:239:25
status: NEW240 Cross-linking of mutants I160C/ F904C and F163C/R905C with oxidant however, inhibited more than 90% of the activity.
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ABCB1 p.Phe163Cys 25987565:240:42
status: NEW