ABCB1 p.Leu797Ala
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PMID: 24275649
[PubMed]
Loo TW et al: "Locking intracellular helices 2 and 3 together inactivates human P-glycoprotein."
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Sentence
Comment
12
Mutation of hydrophobic residues (F793A, L797A, L814A, and L818A) flanking IH3 also inhibited maturation.
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ABCB1 p.Leu797Ala 24275649:12:41
status: NEW81 Point mutations to the residues flanking IH3 (L797A, Fig. 1B) along with F793A, L814A, and L818A (data not shown) also inhibited maturation so that the 150-kDa protein was the major product.
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ABCB1 p.Leu797Ala 24275649:81:46
status: NEW86 Because the mutations F793A, L797A, L814A, and L818A in the flanking regions of IH3 also inhibited maturation, these mutants were also expressed in the presence of cyclosporine A to test for rescue.
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ABCB1 p.Leu797Ala 24275649:86:29
status: NEW88 Mutants F793A, L797A, and L818A were less efficiently rescued as they yielded about equivalent levels of mature 170-kDa and immature 150-kDa P-gp in the presence of cyclosporine A (Fig. 2B).
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ABCB1 p.Leu797Ala 24275649:88:15
status: NEW102 A52-tagged WT P-gp, mutants W803A and F804A (A), or IH3 flanking mutants (F793A, L797A, L814A, and L818A) (B) were expressed in HEK293cellsintheabsence(afa;)orpresence(af9;)of5òe;M cyclosporineA(Cyclo) for 18 h, and whole cell extracts were subjected to immunoblot analysis.
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ABCB1 p.Leu797Ala 24275649:102:81
status: NEW185 In support of the prediction that Trp803 and Phe804 form a hydrophobic pocket with these residues, alanine-scanning mutagenesis of the 793-797 and 814-818 regions showed that only the F793A, L797A, L814A, and L818A mutations inhibited maturation (Fig. 2B).
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ABCB1 p.Leu797Ala 24275649:185:191
status: NEW