ABCB1 p.Phe1086*
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PMID: 23733192
[PubMed]
Loo TW et al: "Human P-glycoprotein contains a greasy ball-and-socket joint at the second transmission interface."
No.
Sentence
Comment
212
A, F1086X mutants were expressed in the absence of drug substrates. B, processing mutants F1086D and F1086R were expressed in the presence (af9;) or absence (afa;) of cyclosporine A (Cyclo), and whole cell SDS extracts were subjected to immunoblot analysis.
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ABCB1 p.Phe1086* 23733192:212:3
status: NEW213 The positions of mature (170 kDa) and immature (150 kDa) P-gps are indicated. C, ATPase activities of histidine-tagged wild-type P-gp and F1086X mutants were measured in the presence of verapamil.
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ABCB1 p.Phe1086* 23733192:213:138
status: NEW
PMID: 25987565
[PubMed]
Loo TW et al: "The Transmission Interfaces Contribute Asymmetrically to the Assembly and Activity of Human P-glycoprotein."
No.
Sentence
Comment
175
These mutants and A52-tagged mutants F1086X (X afd; A, L, W, or R) (23) were transiently expressed in HEK 293 cells and whole cell SDS extracts subjected to immunoblot analysis.
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ABCB1 p.Phe1086* 25987565:175:37
status: NEW179 Histidine-tagged versions of the L443X (X afd; A, S, F, or R) and F1086X (X afd; A, L, W, or R) mutants were expressed in HEK 293 cells in the presence of cyclosporine A to promote maturation of the mutants.
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ABCB1 p.Phe1086* 25987565:179:69
status: NEW