ABCB1 p.Phe1086Asp
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PMID: 23733192
[PubMed]
Loo TW et al: "Human P-glycoprotein contains a greasy ball-and-socket joint at the second transmission interface."
No.
Sentence
Comment
175
Fig. 5A shows that the F1086D or F1086R mutations inhibited maturation of P-gp.
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ABCB1 p.Phe1086Asp 23733192:175:23
status: NEW177 Mutants F1086D and F1086R could still be rescued if they were expressed in the presence of a drug substrate (Fig. 5B).
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ABCB1 p.Phe1086Asp 23733192:177:8
status: NEW179 Mutants F1086D and F1086R were first expressed in the presence of cyclosporine A to promote maturation before isolating the histidine-tagged protein.
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ABCB1 p.Phe1086Asp 23733192:179:8
status: NEW212 A, F1086X mutants were expressed in the absence of drug substrates. B, processing mutants F1086D and F1086R were expressed in the presence (af9;) or absence (afa;) of cyclosporine A (Cyclo), and whole cell SDS extracts were subjected to immunoblot analysis.
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ABCB1 p.Phe1086Asp 23733192:212:90
status: NEW214 Histidine-tagged mutants F1086D and F1086R were first expressed in the presence of cyclosporine A to promote maturation of P-gp before isolation of the protein by nickel-chelate chromatography. Each value is the mean afe; S.D. (n afd; 3).
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ABCB1 p.Phe1086Asp 23733192:214:25
status: NEW