ABCB1 p.Tyr1087Ala
[switch to full view]Comments [show]
None has been submitted yet.
PMID: 23733192
[PubMed]
Loo TW et al: "Human P-glycoprotein contains a greasy ball-and-socket joint at the second transmission interface."
No.
Sentence
Comment
126
To test whether replacement of Phe-1086 with a small amino acid would affect maturation, mutants F1086A or Y1087A in the wild-type background were constructed and expressed in HEK 293 cells.
X
ABCB1 p.Tyr1087Ala 23733192:126:107
status: NEW127 Fig. 3D shows that the Y1087A but not the F1086A mutation inhibited P-gp maturation (Fig. 3D).
X
ABCB1 p.Tyr1087Ala 23733192:127:23
status: NEW
PMID: 24275649
[PubMed]
Loo TW et al: "Locking intracellular helices 2 and 3 together inactivates human P-glycoprotein."
No.
Sentence
Comment
11
Mutants Y1087A and Y1087L, but not Y1087F, were misprocessed, and all inhibited ATPase activity.
X
ABCB1 p.Tyr1087Ala 24275649:11:8
status: NEW148 To test whether Tyr1087 was important for folding or mediating drug stimulation of ATPase activity, we tested the effect of Y1087A, Y1087L, and Y1087F mutations.
X
ABCB1 p.Tyr1087Ala 24275649:148:124
status: NEW149 The A52-tagged mutants were expressed in HEK 293 cells in the absence or presence of cyclosporine A, and samples of whole cell SDS extracts were subjected to immunoblot analysis. Fig. 5A shows that the mature 170-kDa protein was the major product in mutant Y1087F, whereas the 150-kDa immature protein was the major product in mutants Y1087A and Y1087L.
X
ABCB1 p.Tyr1087Ala 24275649:149:335
status: NEW150 Both mutants Y1087A and Y1087L, however, could be rescued when they were expressed in the presence of cyclosporine A (Fig. 5A).
X
ABCB1 p.Tyr1087Ala 24275649:150:13
status: NEW153 It was found that the Y1087A and Y1087L mutations reduced activity by over 90% (Fig. 5B).
X
ABCB1 p.Tyr1087Ala 24275649:153:22
status: NEW164 A, A52-tagged Y1087A (A), Y1087F (F), and Y1087L (L) mutants were expressed in the absence (afa;) or presence (af9;) of 5 òe;M cyclosporine A (Cyclo).
X
ABCB1 p.Tyr1087Ala 24275649:164:14
status: NEW166 The positions of mature (170-kDa) and immature (150-kDa) P-gps are indicated. B, histidine-tagged WT, Y1087A (A), Y1087F (F), and Y1087L (L) P-gp were expressed in HEK 293 cells and isolated by nickel-chelate chromatography.
X
ABCB1 p.Tyr1087Ala 24275649:166:102
status: NEW167 Mutants Y1087A and Y1087L were expressed in the presence of cyclosporine A to promote maturation.
X
ABCB1 p.Tyr1087Ala 24275649:167:8
status: NEW171 Mutations of Tyr1087 to Ala or Leu severely reduced both maturation and activity of P-gp, and even the small change of replacing Tyr with Phe caused about a 70% reduction in activity.
X
ABCB1 p.Tyr1087Ala 24275649:171:13
status: NEW