ABCC7 p.Lys1250Thr
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PMID: 7543023
[PubMed]
Gunderson KL et al: "Conformational states of CFTR associated with channel gating: the role ATP binding and hydrolysis."
No.
Sentence
Comment
57
These NBF1 and NBF2 mutants, harboring the individual mutations K464A and K1250A, K1250G, K1250M, or K1250T, respectively, were expressed in HEK cells and reconstituted into planar lipid bilayers from which single-channel currents were recorded (Figures 3A and 3B).
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ABCC7 p.Lys1250Thr 7543023:57:101
status: NEW76 (B)Single-channelrecordsof K1250A,K1250G, K1250M, and K1250T filtered at 50 Hz under standard cis bath conditions.
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ABCC7 p.Lys1250Thr 7543023:76:54
status: NEW214 Polymerase Chain Reaction Megaprimer Mutagenesis The following site-directed mutants were constructed by using the megaprimer polymerase chain reaction (PCR)-based mutagenesis protocol (Landt et al., 1990; Sarkar and Sommer, 1990): K464A, K1250A, K1250G, K1250T, and GG1247, 1249DE.
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ABCC7 p.Lys1250Thr 7543023:214:255
status: NEW215 Polymerase Chain Reaction Megaprimer Mutagenesis The following site-directed mutants were constructed by using the megaprimer polymerase chain reaction (PCR)-based mutagenesis protocol (Landt et al., 1990; Sarkar and Sommer, 1990): K464A, K1250A, K1250G, K1250T, and GG1247, 1249DE.
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ABCC7 p.Lys1250Thr 7543023:215:255
status: NEW