ABCMdb

ABCC7 p.Thr757Ser

None has been submitted yet.

Publications

PMID: 10692317 [PubMed] Xie J et al: "Conformation, independent of charge, in the R domain affects cystic fibrosis transmembrane conductance regulator channel openings."

No. Sentence Comment

33 Site-specific mutations were constructed following the manufacturer`s instructions using the following three mutagenesis oligonucleotides (showed 5Ј to 3Ј, with mutated base underlined): P740A, G CTC AGA ATC TGC TAC TAA GGA CAG C (RsaI enzyme restriction site is destroyed); P750A, G GCT GAT GCG AGC CAG TAT CGC CTC (BsrI site is created); P759A/T757S, C CTG AAG CGT GGC CGG AGA GCT GAT (BsaHI site is created and BsrI site is destroyed). Login to comment
35 Although mutation was intended to affect only proline residues, upon sequencing, an additional conservative mutation, T757S, was noted. Login to comment
36 Subsequently, the individual prolines were mutated in sequence, with and without the T757S mutation, and their function was examined. Login to comment
37 No difference in channel activity was produced for any mutant by the T757S substitution. Login to comment
38 Data presented in this paper for mutants containing P759A mutation are for constructs that also contain the unexpected T757S mutation. Login to comment
34 Site-specific mutations were constructed following the manufacturer`s instructions using the following three mutagenesis oligonucleotides (showed 5b18; to 3b18;, with mutated base underlined): P740A, G CTC AGA ATC TGC TAC TAA GGA CAG C (RsaI enzyme restriction site is destroyed); P750A, G GCT GAT GCG AGC CAG TAT CGC CTC (BsrI site is created); P759A/T757S, C CTG AAG CGT GGC CGG AGA GCT GAT (BsaHI site is created and BsrI site is destroyed). Login to comment
39 Data presented in this paper for mutants containing P759A mutation are for constructs that also contain the unexpected T757S mutation. Login to comment