ABCC7 p.Thr1478*
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PMID: 15463893
[PubMed]
Braunstein GM et al: "Purinergic signaling underlies CFTR control of human airway epithelial cell volume."
No.
Sentence
Comment
119
IB3-1 cells express the DF508-CFTR and W1282X-CFTR mutations endogenously [35,49].
X
ABCC7 p.Thr1478* 15463893:119:40
status: NEW121 No CFTR protein was detectable in ''mock controls``, while band B ''core glycosylated`` (130-140 kDa) and band C ''maturely glycosylated`` (160-180 kDa) forms of CFTR were found for WT-CFTR, T1478X (also DTRL)-CFTR, S1455X-CFTR, and G551D-CFTR [9,51,52].
X
ABCC7 p.Thr1478* 15463893:121:191
status: NEW122 S1455X-CFTR showed a similar profile to T1478X-CFTR (data not shown).
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ABCC7 p.Thr1478* 15463893:122:40
status: NEW172 When compared to empty vector, WT-CFTR and T1478X-CFTR were able to fully recover their cell volume after hypotonic cell swelling Fig. 3.
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ABCC7 p.Thr1478* 15463893:172:43
status: NEW204 The IB3-1 cells transiently transfected with WT- and T1478X-CFTR showed an increase in chloride permeability and increased rate of ClÀ release in response to 25% hypotonic shock (Fig. 5B).
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ABCC7 p.Thr1478* 15463893:204:53
status: NEW216 IB3-1 cells expressing WT- and T1478X-CFTR showed a marked increase in magnitude of chloride efflux as well as increased rate of chloride release (n = 3-4 experiments for each construct, asterisk indicates P < 0.01 versus empty vector control, cross indicates P < 0.05 versus empty vector control).
X
ABCC7 p.Thr1478* 15463893:216:31
status: NEW118 No CFTR protein was detectable in ''mock controls``, while band B ''core glycosylated`` (130-140 kDa) and band C ''maturely glycosylated`` (160-180 kDa) forms of CFTR were found for WT-CFTR, T1478X (also DTRL)-CFTR, S1455X-CFTR, and G551D-CFTR [9,51,52].
X
ABCC7 p.Thr1478* 15463893:118:191
status: NEW167 When compared to empty vector, WT-CFTR and T1478X-CFTR were able to fully recover their cell volume after hypotonic cell swelling Fig. 3.
X
ABCC7 p.Thr1478* 15463893:167:43
status: NEW199 The IB3-1 cells transiently transfected with WTand T1478X-CFTR showed an increase in chloride permeability and increased rate of Cl release in response to 25% hypotonic shock (Fig. 5B).
X
ABCC7 p.Thr1478* 15463893:199:51
status: NEW211 IB3-1 cells expressing WTand T1478X-CFTR showed a marked increase in magnitude of chloride efflux as well as increased rate of chloride release (n = 3-4 experiments for each construct, asterisk indicates P < 0.01 versus empty vector control, cross indicates P < 0.05 versus empty vector control).
X
ABCC7 p.Thr1478* 15463893:211:29
status: NEW