ABCMdb

ABCC7 p.Gln237Leu

None has been submitted yet.

Publications

PMID: 17949679 [PubMed] Wehbi H et al: "Positional dependence of non-native polar mutations on folding of CFTR helical hairpins."

No. Sentence Comment

95 In contrast, replacement of wild type Q237 in TM4 with the non-polar Leu residue (mutant Q237L) creates a slower migrating hairpin vs. wild type (Fig. 1a), suggesting that some pre-existing inter-helical interactions - conceivably attributable to a network of H-bonding interactions - have been removed. Login to comment
96 The helix-helix interactions influenced by polar mutants among the positions studied are summarized in Fig. 1b, where it is seen that constructs involving I231 and V232 with combinations of TM4 D-mutations, as well as various combinations of Q207N with D and E mutants in TM4, migrate between 7 and 20% faster than the WT construct. Login to comment
123 (a) Relative migration rates of wild type CFTR TM3/4 and mutants Q237L and Q207N-V232E. Login to comment
124 (a) Relative migration rates of wild type CFTR TM3/4 and mutants Q237L and Q207N-V232E. Login to comment

PMID: 15209503 [PubMed] Choi MY et al: "Non-native interhelical hydrogen bonds in the cystic fibrosis transmembrane conductance regulator domain modulated by polar mutations."

No. Sentence Comment

118 Figure 5 displays the comparison of the migration differences on SDS-PAGE between six X f D mutants and their Q237L counterparts in the CFTR TM3/4 construct. Login to comment
119 Our results indicate a discrete role for Q237, viz., the double mutants F224D/ Q237L, A238D/Q237L and G239D/Q237Lsin which the D locus is nearer to the Nor C-terminus of the TM4 helixs returned to the wt migration rate. Login to comment
120 D mutants in mid-TM4, i.e., V232D/Q237L and L230D/Q237L, continued to migrate faster than the wt, but were clearly retarded vs their D counterparts. Login to comment
141 FIGURE 5: Comparison of relative migration rates on SDS-PAGE (NuPAGE MES gel system) for X f D mutants and selected XD/ Q237L mutants in CFTR TM3/4 constructs. Login to comment
178 From the results (Figures 5 and 7), we found that double mutants where D is relatively distant from Q207 in TM3 (F224D/ Q237L, A238D/Q237L, and G239D/Q237L) returned to the wt migration rate, whereas the other double mutants (V232D/ Q237L and L230D/Q237L) migrated more slowly than the corresponding D mutants, but still significantly faster than the wt construct. Login to comment