ABCC1 p.Asp572Asn
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PMID: 21625534
[PubMed]
Johnson JS et al: "AAV exploits subcellular stress associated with inflammation, endoplasmic reticulum expansion, and misfolded proteins in models of cystic fibrosis."
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283
Materials and Methods Cell culture HeLa cells were maintained at 37uC and 5% CO2 in Dulbecco`s modified Eagle`s medium (DMEM) that was supplemented with 10% heat-inactivated fetal calf serum, 100 U/ml penicillin, and 100 g/ml streptomycin. BHK-21 cells were grown at 37uC and 5% CO2 in Dulbecco`s modified Eagle`s medium (DMEM) and Ham`s F12 medium (50:50) that was supplemented with 5% heat-inactivated fetal calf serum, 100 U/ml penicillin, and 100 g/ml streptomycin. BHK-21 clones expressing wild-type CFTR, CFTR-DF508, CFTR-G551D, CFTR-D572N, CFTR- 1410X, wild-type MRP1, or MRP1-DF728 were maintained in selection medium containing methotrexate (250 mg/ml).
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ABCC1 p.Asp572Asn 21625534:283:540
status: NEW