ABCC7 p.Glu92Cys
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PMID: 21746847
[PubMed]
Wang W et al: "Alignment of transmembrane regions in the cystic fibrosis transmembrane conductance regulator chloride channel pore."
No.
Sentence
Comment
66
The one exception was E92C, which failed to generate any measureable current, even after multiple attempts with two independently constructed mutant cDNAs.
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ABCC7 p.Glu92Cys 21746847:66:22
status: NEW118 Note that no currents were recorded from patches excised from cells transfected with E92C cDNA (see Results).
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ABCC7 p.Glu92Cys 21746847:118:85
status: NEW
PMID: 23442957
[PubMed]
Gao X et al: "Cysteine scanning of CFTR's first transmembrane segment reveals its plausible roles in gating and permeation."
No.
Sentence
Comment
83
We could only obtain microscopic current with E92C-CFTR probably due to a poor expression, but the application of MTSES decreased the single-channel amplitude of this construct (see below).
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ABCC7 p.Glu92Cys 23442957:83:46
status: NEW165 State-dependent modification of E92C-, K95C-, Q98C-, and L102C-CFTR The observation that MTSET modification of Q98C and L102C alters CFTR gating suggests that TM1 indeed participates in gating motions of CFTR.
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ABCC7 p.Glu92Cys 23442957:165:32
status: NEW171 As this series of experiments requires macroscopic currents, we could not test the E92C mutants, which express poorly (see Discussion for details).
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ABCC7 p.Glu92Cys 23442957:171:83
status: NEW250 Although we were not able to measure the modification rate for E92C because of poor expression, in five patches yielding microscopic current, MTSES appears to modify the introduced cysteine when the channel is opened (see Fig. S7).
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ABCC7 p.Glu92Cys 23442957:250:63
status: NEW