ABCC1 p.Pro104Ala
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PMID: 12948592
[PubMed]
Ito K et al: "Mutation of proline residues in the NH(2)-terminal region of the multidrug resistance protein, MRP1 (ABCC1): effects on protein expression, membrane localization, and transport function."
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Comment
3
All singly substituted MRP1-Pro mutants could be expressed in HeLa cells, except MRP1-P104A.
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ABCC1 p.Pro104Ala 12948592:3:86
status: NEW46 For the single substitutions of Pro with Ala in MSD1, the following sense mutagenic primers were used (substituted nucleotides are underlined): MRP1-P42A (5V-C GTG TGG GTG GCT TGT TTT TAC CTC TGG-3V), MRP1-P51A (5V-G GCC TGT TTC GCC TTC TAC TTC C-3V), MRP1-P69A (5V-CAG ATG ACA GCT CTC AAC-3V), MRP1-P104A (5V-C ATA TTC CTG GCC GCA GTG TTT CTG G-3V), MRP1-P110A (5V-GT TTC TGG TCA GCG CAA CTC TCT TGG-3V).
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ABCC1 p.Pro104Ala 12948592:46:300
status: NEWX
ABCC1 p.Pro104Ala 12948592:46:306
status: NEW103 Despite multiple attempts, stable HeLa cell lines expressing MRP1-P104A and MRP1-P104/110A could not be established.
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ABCC1 p.Pro104Ala 12948592:103:66
status: NEW