ABCC11 p.Asn844Gln
[switch to full view]Comments [show]
None has been submitted yet.
PMID: 19383836
[PubMed]
Toyoda Y et al: "Earwax, osmidrosis, and breast cancer: why does one SNP (538G>A) in the human ABC transporter ABCC11 gene determine earwax type?"
No.
Sentence
Comment
73
The resulting expression construct [ABCC11 WT-pcDNA3.1/ Hygro(-)] was used as the template for site-directed mutagenesis to obtain ABCC11 variants, i.e., N838Q, N844Q, and N838Q/N844Q.
X
ABCC11 p.Asn844Gln 19383836:73:161
status: NEWX
ABCC11 p.Asn844Gln 19383836:73:178
status: NEW75 To generate the N838Q/N844Q variant, ABCC11 N838Q- pcDNA3.1/Hygro(-) was used as the template.
X
ABCC11 p.Asn844Gln 19383836:75:22
status: NEW165 Substitution of both Asn838 and Asn844 to Gln residues completely diminished N-linked glycosylation of the ABCC11 WT (Fig. 5B), demonstrating that these two Asn residues are N-linked glycosylation sites in the ABCC11 WT protein.
X
ABCC11 p.Asn844Gln 19383836:165:32
status: NEW198 B) Effect of substitution of Asn838 and Asn844 to Gln on N-linked glycosylation status of ABCC11.
X
ABCC11 p.Asn844Gln 19383836:198:40
status: NEW74 The resulting expression construct [ABCC11 WT-pcDNA3.1/ Hygro(afa;)] was used as the template for site-directed mutagenesis to obtain ABCC11 variants, i.e., N838Q, N844Q, and N838Q/N844Q.
X
ABCC11 p.Asn844Gln 19383836:74:167
status: NEWX
ABCC11 p.Asn844Gln 19383836:74:184
status: NEW76 To generate the N838Q/N844Q variant, ABCC11 N838Q- pcDNA3.1/Hygro(afa;) was used as the template.
X
ABCC11 p.Asn844Gln 19383836:76:22
status: NEW166 Substitution of both Asn838 and Asn844 to Gln residues completely diminished N-linked glycosylation of the ABCC11 WT (Fig. 5B), demonstrating that these two Asn residues are N-linked glycosylation sites in the ABCC11 WT protein.
X
ABCC11 p.Asn844Gln 19383836:166:32
status: NEW199 B) Effect of substitution of Asn838 and Asn844 to Gln on N-linked glycosylation status of ABCC11.
X
ABCC11 p.Asn844Gln 19383836:199:40
status: NEW