ABCC4 p.Lys1081Asn
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PMID: 18300232
[PubMed]
Janke D et al: "6-mercaptopurine and 9-(2-phosphonyl-methoxyethyl) adenine (PMEA) transport altered by two missense mutations in the drug transporter gene ABCC4."
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165
Moreover, a mutation was introduced into the critical WalkerA motif of MRP4 ATP-binding site 2 (K1081N) to provide an additional, negative control for the efflux experiments.
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ABCC4 p.Lys1081Asn 18300232:165:96
status: NEW167 In fact, substrate efflux in the absence or presence (Supplementary Fig. S4) of extracellular added unlabeled 500 mM 6-MP or 5 mM PMEA from oocytes expressing the MRP4(K1081N) mutant was not different from efflux of control oocytes, verifying that a functional WalkerA motif is necessary for MRP4, and providing a nonfunctional MRP4 control.
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ABCC4 p.Lys1081Asn 18300232:167:168
status: NEW183 All other variants including MRP4 (K1081N) had a fluorescence intensity similar to wild-type MRP4.
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ABCC4 p.Lys1081Asn 18300232:183:35
status: NEW