ABCC4 p.Trp995Ala
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PMID: 22542979
[PubMed]
Wittgen HG et al: "Phenylalanine 368 of multidrug resistance-associated protein 4 (MRP4/ABCC4) plays a crucial role in substrate-specific transport activity."
No.
Sentence
Comment
46
Site-directed mutagenesis of MRP4 and generation of expression vectors and baculovirus The previously described Gateway entry vector containing the human MRP4 coding sequence [8] was used as a template for site-directed mutagenesis of the following amino acids: F368W, F368Y, F368A, W995F, W995Y, W995A, R998S, R998K, R998Y, and R998L.
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ABCC4 p.Trp995Ala 22542979:46:297
status: NEW115 Substitution of Phe368 and Trp995 with Ala, and all amino acid substitutions of Arg998 almost completely disrupted transport activity for all substrates tested.
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ABCC4 p.Trp995Ala 22542979:115:27
status: NEW122 As F368A, W995A, and all mutations of Arg998 completely disrupted the transport activity of MRP4, it was not possible to include these mutant proteins into the kinetic analysis.
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ABCC4 p.Trp995Ala 22542979:122:10
status: NEW47 Site-directed mutagenesis of MRP4 and generation of expression vectors and baculovirus The previously described Gateway entry vector containing the human MRP4 coding sequence [8] was used as a template for site-directed mutagenesis of the following amino acids: F368W, F368Y, F368A, W995F, W995Y, W995A, R998S, R998K, R998Y, and R998L.
X
ABCC4 p.Trp995Ala 22542979:47:297
status: NEW