ABCC2 p.Leu23Ser
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PMID: 11839251
[PubMed]
Connolly L et al: "A new monoclonal antibody, P2A8(6), that specifically recognizes a novel epitope on the multidrug resistance-associated protein 1 (MRP1), but not on MRP2 nor MRP3."
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Sentence
Comment
43
Control cell lines The multidrug resistant nonsmall cell lung carcinoma cell line, COR-L23R (made resistant by exposure to increasing concentrations of adriamycin(26)) and its drug-sensitive counterpart, COR-L23S, were used as control cells for the presence/absence of MRP1.
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ABCC2 p.Leu23Ser 11839251:43:208
status: NEW44 Overexpression of MRP1 in COR-L23R cells compared with the parental COR-L23S had been confirmed in previous studies using other commercially available antibodies raised against MRP1 (MRPr1, MRPm5, MRPm6,(15) QCRL-1, and QCRL-3(16)) (Alexis Biochemicals, San Diego, CA).
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ABCC2 p.Leu23Ser 11839251:44:72
status: NEW77 P2A8(6) also detects a very faint 190-kDa band on preparationsfrom drug-sensitivecells (COR-L23S) and non- transfectedcells (2008 parental)indicatingan intrinsic basal expression of MRP1.
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ABCC2 p.Leu23Ser 11839251:77:92
status: NEW110 A very low basal level of MRP1 is detected in the sensitive COR-L23S cells.
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ABCC2 p.Leu23Ser 11839251:110:64
status: NEW115 MRP2 is undetectable in the COR-L23R, COR-L23S, HL60ADR, HL60S, DLKP, RPMI 2650, and RPMI 2650 taxol-selected cells.
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ABCC2 p.Leu23Ser 11839251:115:42
status: NEW126 Neither MAb detected MRP1 expression in the COR-L23S, HL60S, DLKP, DLKPA, or RPMI 2650 taxol-selected cell lines.
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ABCC2 p.Leu23Ser 11839251:126:48
status: NEW134 SUMMARY OF MRP1, MRP2, AND MRP3 PROTEIN EXPRESSION LEVELS AS DETERMINED BY WESTERN BLOT ANALYSIS Protein expression levels (as determined by Western blot analysis) MRPr1 M2III-6 M3II-21 Cell line P2A8(6) (MRP1) (MRP2) (MRP3) COR-L23R 1 1 - - COR-L23S 1/2 1/2 - - HL60ADR 1 1 - - HL60S - - - - DLKP - - - - DLKPA - - - - A549 1 1 1 - RPMI 2650 - 1/2 - - Parental RPMI 2650 - - - - Taxol-selected RPMI 2650 1 1 1 - Melphalan-selected Key: -: Undetectable levels; 1: Over-expression; and 1/2: Very low basal levels.
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ABCC2 p.Leu23Ser 11839251:134:246
status: NEW141 MAb P2A8(6) did not detect overexpressionof MRP1 in the sensitiveCOR-L23S, 2008 parental,2008 MRP2, or 2008 MRP3 cell lines but like MRPr1, P2A8(6) did detect a very low basal level of staining in these cell lines.
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ABCC2 p.Leu23Ser 11839251:141:69
status: NEW