ABCMdb

ABCC4 p.Arg375Lys

None has been submitted yet.

Publications

PMID: 18612080 [PubMed] El-Sheikh AA et al: "Functional role of arginine 375 in transmembrane helix 6 of multidrug resistance protein 4 (MRP4/ABCC4)."

No. Sentence Comment

61 Twelve mutants of the human MRP4 were generated: FF/L- (F368L and F369-), ERE/ SSQ (E374S, R375S, and E378Q), FFERE/L-SSQ (F368L, F369-, E374S, R375S, and E378Q), F368L, F369-, E374S, R375S, R375A, R375K, R375E, E378Q, and R998A. Login to comment
126 Mean values Ϯ S.E. of three enzyme preparations are shown. Fig. 6. Western blot analysis (A), 0.5 ␮M [3 H]MTX (B), and 1 ␮M [3 H]cGMP (C) transport activity of wild-type, R375A, R375K, R375E, and R998A MRP4 transporter proteins. Login to comment
127 A, top, Western blot of membrane vesicles isolated from HEK293 cells overexpressing MRP4 or MRP4 mutants R375A, R375K, R375E, and R998A, as well as negative control detected by polyclonal anti-human MRP4 (representative of three). Login to comment
157 Concentration-dependent uptake of [3 H]MTX and [3 H]cGMP in membrane vesicles expressing human MRP4 mutants. Control (Œ), wild-type (f), R375A (F), R375K (E), R375E (छ), and R998A (x) MRP4 membrane vesicles were incubated with [3 H]MTX (top) or [3 H]cGMP (bottom) concentrations indicated in the figure. Login to comment
165 Figure 6A shows an equal level of protein expression of wild type as well as R375A, R375K, R375E, and R988A mutants. Login to comment
172 The Km values of wild-type MRP4 and R375K were 230 Ϯ 90 and 250 Ϯ 20 ␮M for MTX and 610 Ϯ 70 and 640 Ϯ 60 ␮M for cGMP, respectively. Login to comment
173 Thus, R375K retained substrate affinity comparable with the wild type for both MTX and cGMP. Login to comment
244 3 and 4 MRP4 230 Ϯ 90 280 Ϯ 60 610 Ϯ 70 370 Ϯ 30 164 Ϯ 4 R375S 720 Ϯ 320 270 Ϯ 120 610 Ϯ 80 270 Ϯ 70 470 Ϯ 70 Data from Fig. 4 MRP4 230 Ϯ 90 250 Ϯ 20 610 Ϯ 70 420 Ϯ 10 R375K 250 Ϯ 20 190 Ϯ 10 640 Ϯ 60 420 Ϯ 20 substrate-binding site. Login to comment