ABCC4 p.Arg375Ala
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PMID: 18612080
[PubMed]
El-Sheikh AA et al: "Functional role of arginine 375 in transmembrane helix 6 of multidrug resistance protein 4 (MRP4/ABCC4)."
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Sentence
Comment
215
Deletion of the hydroxyl group of MRP1 residue 605 (S605A), corresponding to R375A in MRP4, decreased resistance to vincristine, etoposide (VP-16), and doxorubicin (Zhang et al., 2004).
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ABCC4 p.Arg375Ala 18612080:215:77
status: NEW61 Twelve mutants of the human MRP4 were generated: FF/L- (F368L and F369-), ERE/ SSQ (E374S, R375S, and E378Q), FFERE/L-SSQ (F368L, F369-, E374S, R375S, and E378Q), F368L, F369-, E374S, R375S, R375A, R375K, R375E, E378Q, and R998A.
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ABCC4 p.Arg375Ala 18612080:61:191
status: NEW126 Mean values Ϯ S.E. of three enzyme preparations are shown. Fig. 6. Western blot analysis (A), 0.5 M [3 H]MTX (B), and 1 M [3 H]cGMP (C) transport activity of wild-type, R375A, R375K, R375E, and R998A MRP4 transporter proteins.
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ABCC4 p.Arg375Ala 18612080:126:191
status: NEW127 A, top, Western blot of membrane vesicles isolated from HEK293 cells overexpressing MRP4 or MRP4 mutants R375A, R375K, R375E, and R998A, as well as negative control detected by polyclonal anti-human MRP4 (representative of three).
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ABCC4 p.Arg375Ala 18612080:127:105
status: NEW157 Concentration-dependent uptake of [3 H]MTX and [3 H]cGMP in membrane vesicles expressing human MRP4 mutants. Control (Œ), wild-type (f), R375A (F), R375K (E), R375E (छ), and R998A (x) MRP4 membrane vesicles were incubated with [3 H]MTX (top) or [3 H]cGMP (bottom) concentrations indicated in the figure.
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ABCC4 p.Arg375Ala 18612080:157:143
status: NEW165 Figure 6A shows an equal level of protein expression of wild type as well as R375A, R375K, R375E, and R988A mutants.
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ABCC4 p.Arg375Ala 18612080:165:77
status: NEW170 The transport activities of R375A, R375E, and R988A for MTX and cGMP did not differ from that of the negative control.
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ABCC4 p.Arg375Ala 18612080:170:28
status: NEW188 Nevertheless, several MRP4 mutants (FF/L-, ERE/SSQ, FFERE/L-SSQ, F368L, F369-, E374S, R375A, R375E, E378Q, and R998A) showed significantly diminished transport activity of either MTX or cGMP compared with that of wild-type MRP4.
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ABCC4 p.Arg375Ala 18612080:188:86
status: NEW221 When this charge is removed (R375S), the MTX affinity decreases or transport activity is absent (R375A and R375E).
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ABCC4 p.Arg375Ala 18612080:221:97
status: NEW223 As soon as this hydroxyl group is removed (R375A) or replaced with a (negatively charged) acidic group (R375E), transport of cGMP is absent.
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ABCC4 p.Arg375Ala 18612080:223:43
status: NEW