ABCC4 p.Arg998Ala
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PMID: 18612080
[PubMed]
El-Sheikh AA et al: "Functional role of arginine 375 in transmembrane helix 6 of multidrug resistance protein 4 (MRP4/ABCC4)."
No.
Sentence
Comment
230
Moreover, alanine substitution of Arg1249 in MRP1 (also corresponding to R998A) impaired MRP1-mediated LTC4 transport and reduced vincristine resistance (Ren et al., 2002).
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ABCC4 p.Arg998Ala 18612080:230:73
status: NEW61 Twelve mutants of the human MRP4 were generated: FF/L- (F368L and F369-), ERE/ SSQ (E374S, R375S, and E378Q), FFERE/L-SSQ (F368L, F369-, E374S, R375S, and E378Q), F368L, F369-, E374S, R375S, R375A, R375K, R375E, E378Q, and R998A.
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ABCC4 p.Arg998Ala 18612080:61:223
status: NEW126 Mean values Ϯ S.E. of three enzyme preparations are shown. Fig. 6. Western blot analysis (A), 0.5 M [3 H]MTX (B), and 1 M [3 H]cGMP (C) transport activity of wild-type, R375A, R375K, R375E, and R998A MRP4 transporter proteins.
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ABCC4 p.Arg998Ala 18612080:126:216
status: NEW127 A, top, Western blot of membrane vesicles isolated from HEK293 cells overexpressing MRP4 or MRP4 mutants R375A, R375K, R375E, and R998A, as well as negative control detected by polyclonal anti-human MRP4 (representative of three).
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ABCC4 p.Arg998Ala 18612080:127:130
status: NEW157 Concentration-dependent uptake of [3 H]MTX and [3 H]cGMP in membrane vesicles expressing human MRP4 mutants. Control (Œ), wild-type (f), R375A (F), R375K (E), R375E (छ), and R998A (x) MRP4 membrane vesicles were incubated with [3 H]MTX (top) or [3 H]cGMP (bottom) concentrations indicated in the figure.
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ABCC4 p.Arg998Ala 18612080:157:186
status: NEW188 Nevertheless, several MRP4 mutants (FF/L-, ERE/SSQ, FFERE/L-SSQ, F368L, F369-, E374S, R375A, R375E, E378Q, and R998A) showed significantly diminished transport activity of either MTX or cGMP compared with that of wild-type MRP4.
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ABCC4 p.Arg998Ala 18612080:188:111
status: NEW
PMID: 22542979
[PubMed]
Wittgen HG et al: "Phenylalanine 368 of multidrug resistance-associated protein 4 (MRP4/ABCC4) plays a crucial role in substrate-specific transport activity."
No.
Sentence
Comment
171
In a previous study we already showed that R998A disrupted transport of MTX and cGMP [8].
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ABCC4 p.Arg998Ala 22542979:171:43
status: NEW