ABCB2 p.Gly645Ala
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PMID: 15322097
[PubMed]
Chen M et al: "Functional non-equivalence of ATP-binding cassette signature motifs in the transporter associated with antigen processing (TAP)."
No.
Sentence
Comment
62
Site-directed mutations (L643A, TAP1; G646V, TAP1; G646D, TAP1; L607A, TAP2; G610V, TAP2; G610D, TAP2; S644A/G645A, TAP1; and A608S/A609G, TAP2) were introduced by sequence overlapping PCR using pFastBacDual.TAP1wt or pFastBacDual.TAP2wt as templates.
X
ABCB2 p.Gly645Ala 15322097:62:109
status: NEWX
ABCB2 p.Gly645Ala 15322097:62:513
status: NEW63 The sequences of the sense oligonucleotides were as follows: 5Ј-GCTGGGAGCCAGGCG- TCAGGGGGTCAG-3Ј for TAP1L643A; 5Ј-CAGCTGTCAGGGGTTCAG- CGACAGGCAG-3Ј for TAP1G646V; 5Ј-CAGCTGTCAGGGGATCAGC- GACAGGCAG-3Ј for TAP1G646D; 5Ј-GGAAGCCAGGCGGCTGCGGG- ACAGAAAC-3Ј for TAP2L607A; 5Ј-GCTGGCTGCGGTACAGAAACAA- CGTC-3Ј for TAP2G610V; 5Ј-GCTGGCTGCGGACCAGAAACAACGTC- TG-3Ј for TAP2G610D; 5Ј-GCTGGGAGCCAGCTGGCAGCGGGTCAG- CGACAGGCA-3Ј for TAP1S644A/G645A; and 5Ј-GAAGGGAAGCCAG- CTGTCTGGGGGACAGAAACAACG-3Ј for TAP2A608S/A609G, respectively (mutated bases are underlined).
X
ABCB2 p.Gly645Ala 15322097:63:513
status: NEW61 Site-directed mutations (L643A, TAP1; G646V, TAP1; G646D, TAP1; L607A, TAP2; G610V, TAP2; G610D, TAP2; S644A/G645A, TAP1; and A608S/A609G, TAP2) were introduced by sequence overlapping PCR using pFastBacDual.TAP1wt or pFastBacDual.TAP2wt as templates.
X
ABCB2 p.Gly645Ala 15322097:61:109
status: NEW