ABCA4 p.Thr1979Ala
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PMID: 16533065
[PubMed]
Biswas-Fiss EE et al: "Interaction of the nucleotide binding domains and regulation of the ATPase activity of the human retina specific ABC transporter, ABCR."
No.
Sentence
Comment
74
The following complementary oligonucleotides were used as mutagenic primers to generate the mutant NBD1 and NBD2 proteins: NBD1Pro940Arg (5'-GGT AAA GAT TTT TGA GCG CTG TGG CCG GCC AGC TG-3'), NBD1 Walker A, K969A, T970A (5'-CAA TGG AGC TGG GGC AGC CAC CAC CTT GTC CAT CC-3'), NBD2 Walker A, K1978A, T1979A (5'-GAA TGG TGC CGG CGC AGC AAC CAC ATT CAA GAT GC-3'), and NBD2 R2038W (5'-CTT TAC CTT TAT GCC AGG CTT CGA GGT GTA CCA GC-3').
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ABCA4 p.Thr1979Ala 16533065:74:302
status: NEW111 Lanes (from left to right): 1, NBD1WT; 2, NBD1P940R; 3, NBD1K969A,T970A; 4, NBD2WT; 5, NBD2R2038W; 6, NBD2K1978A,T1979A.
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ABCA4 p.Thr1979Ala 16533065:111:113
status: NEW198 A quantitative evaluation of the interaction between wild-type NBD1 and mutant NBD2K1978A,T1979A in the presence and absence of nucleotides was carried out (Table 1).
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ABCA4 p.Thr1979Ala 16533065:198:90
status: NEW218 On the other hand, the ratio of the experimental versus theoretical ATPase activities of the complex harboring NBD2 Walker A mutations, K1978A, T1979A, in the NBD2 domain was only 0.32, which represents a 68% difference in the experimental versus the theoretical activity, as compared to the 36% difference observed for the wild-type complex.
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ABCA4 p.Thr1979Ala 16533065:218:144
status: NEW225 The ATPase assay was carried out as described under Experimental Procedures at 37 °C for 60 min. A theoretical curve is shown, indicating the results expected if the activity of NBD1K969A,T970A‚ NBD2 were additive (]) of that obtained for the individual domains. Each point represents the average of three independent experiments. FIGURE 7: Effect of NBD2 Walker A mutations K1978A, T1979A on modulation of ATP hydrolysis of the NBD1‚NBD2 protein complex. Protein titration of the ATPase activity of purified NBD2K1978A,T1979A (∆), NBD2WT (2), NBD1WT (0) alone, and the NBD1‚NBD2K1978A,T1979A complex (O).
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ABCA4 p.Thr1979Ala 16533065:225:395
status: NEWX
ABCA4 p.Thr1979Ala 16533065:225:539
status: NEWX
ABCA4 p.Thr1979Ala 16533065:225:619
status: NEW226 The ATPase assay was carried out as described under Experimental Procedures at 37 °C for 60 min. A theoretical curve is shown, indicating the results expected if the activity of NBD1‚NBD2K1978A,T1979A were additive (]) of that obtained for the individual domains. Each point represents the average of three independent experiments. on NBD1.
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ABCA4 p.Thr1979Ala 16533065:226:206
status: NEW