ABCMdb

ABCA3 p.Leu1580Ala

None has been submitted yet.

Publications

PMID: 16959783 [PubMed] Matsumura Y et al: "Characterization and classification of ATP-binding cassette transporter ABCA3 mutants in fatal surfactant deficiency."

No. Sentence Comment

208 In the L1580A and L1580F mutant proteins, which had dramatically impaired ATP hydrolysis, the distance from beta-carbon of Ala-1580 and ␨-carbon of Phe-1580 was extended to 6.3 Å and shortened to 2.2 Å, respectively, compared with that of wild-type protein (Fig. 8, E and G). Login to comment
231 A, 20,000 ϫ g membrane fraction prepared from HEK293 cells stably expressing wild-type (Wt) ABCA3-GFP (lanes 3 and 4), L1580A (lanes 5 and 6), L1580V (lanes 7 and 8), L1580F (lanes 9 and 10), L1580P (lanes 11 and 12), or untransfected HEK293 cells (lanes 1 and 2) was incubated with 10 ␮M 8-azido-[␣-32 P]ATP in the absence (-) or presence (ϩ) of 0.4 mM orthovanadate (Vi) and 3 mM MgCl2 for 10 min at 37 °C. Proteins were photoaffinity-labeled with UV irradiation after removal of unbound ATP, electrophoresed on SDS-PAGE (5%), and transferred to a PVDF membrane. Login to comment
207 In the L1580A and L1580F mutant proteins, which had dramatically impaired ATP hydrolysis, the distance from beta-carbon of Ala-1580 and ॼ-carbon of Phe-1580 was extended to 6.3 &#c5; and shortened to 2.2 &#c5;, respectively, compared with that of wild-type protein (Fig. 8, E and G). Login to comment
230 A, 20,000 afb; g membrane fraction prepared from HEK293 cells stably expressing wild-type (Wt) ABCA3-GFP (lanes 3 and 4), L1580A (lanes 5 and 6), L1580V (lanes 7 and 8), L1580F (lanes 9 and 10), L1580P (lanes 11 and 12), or untransfected HEK293 cells (lanes 1 and 2) was incubated with 10 òe;M 8-azido-[ॷ-32 P]ATP in the absence (afa;) or presence (af9;) of 0.4 mM orthovanadate (Vi) and 3 mM MgCl2 for 10 min at 37 &#b0;C. Proteins were photoaffinity-labeled with UV irradiation after removal of unbound ATP, electrophoresed on SDS-PAGE (5%), and transferred to a PVDF membrane. Login to comment