ABCA3 p.Leu1580Val
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PMID: 16959783
[PubMed]
Matsumura Y et al: "Characterization and classification of ATP-binding cassette transporter ABCA3 mutants in fatal surfactant deficiency."
No.
Sentence
Comment
199
In the L1580V mutant protein, FIGURE 6.
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ABCA3 p.Leu1580Val 16959783:199:7
status: NEW231 A, 20,000 ϫ g membrane fraction prepared from HEK293 cells stably expressing wild-type (Wt) ABCA3-GFP (lanes 3 and 4), L1580A (lanes 5 and 6), L1580V (lanes 7 and 8), L1580F (lanes 9 and 10), L1580P (lanes 11 and 12), or untransfected HEK293 cells (lanes 1 and 2) was incubated with 10 M 8-azido-[␣-32 P]ATP in the absence (-) or presence (ϩ) of 0.4 mM orthovanadate (Vi) and 3 mM MgCl2 for 10 min at 37 °C. Proteins were photoaffinity-labeled with UV irradiation after removal of unbound ATP, electrophoresed on SDS-PAGE (5%), and transferred to a PVDF membrane.
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ABCA3 p.Leu1580Val 16959783:231:149
status: NEW230 A, 20,000 afb; g membrane fraction prepared from HEK293 cells stably expressing wild-type (Wt) ABCA3-GFP (lanes 3 and 4), L1580A (lanes 5 and 6), L1580V (lanes 7 and 8), L1580F (lanes 9 and 10), L1580P (lanes 11 and 12), or untransfected HEK293 cells (lanes 1 and 2) was incubated with 10 òe;M 8-azido-[ॷ-32 P]ATP in the absence (afa;) or presence (af9;) of 0.4 mM orthovanadate (Vi) and 3 mM MgCl2 for 10 min at 37 &#b0;C. Proteins were photoaffinity-labeled with UV irradiation after removal of unbound ATP, electrophoresed on SDS-PAGE (5%), and transferred to a PVDF membrane.
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ABCA3 p.Leu1580Val 16959783:230:149
status: NEW