ABCA4 p.Phe2219Ala
[switch to full view]Comments [show]
None has been submitted yet.
PMID: 15347662
[PubMed]
Fitzgerald ML et al: "ATP-binding cassette transporter A1 contains a novel C-terminal VFVNFA motif that is required for its cholesterol efflux and ApoA-I binding activities."
No.
Sentence
Comment
49
Mutations of the VFVNFA motif of ABCA1 (2215 VFVNFA2220 3 2215 AAAAA- A2220 , 2215 VFVNFA2220 3 2215 VFLYFS2220 , V2215A, F2216A, V2217A, V2217L, N2218A, and F2219A) were generated by overlap PCR.
X
ABCA4 p.Phe2219Ala 15347662:49:158
status: NEW164 To further dissect the motif itself, the contributions of the individual residues within the VFVNFA motif were defined by generating single alanine substitutions (V2215A, F2216A, V2217A, N2218A, and F2219A).
X
ABCA4 p.Phe2219Ala 15347662:164:145
status: NEWX
ABCA4 p.Phe2219Ala 15347662:164:199
status: NEW165 At moderate expression levels in 293 cells, all of the single mutations showed a near complete loss of efflux activity with the exception of the F2219A mutant, whose activity was similar to that of wild type ABCA1 (Fig. 7A).
X
ABCA4 p.Phe2219Ala 15347662:165:145
status: NEW163 To further dissect the motif itself, the contributions of the individual residues within the VFVNFA motif were defined by generating single alanine substitutions (V2215A, F2216A, V2217A, N2218A, and F2219A).
X
ABCA4 p.Phe2219Ala 15347662:163:199
status: NEW