ABCA1 p.Cys23Ser
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PMID: 20656214
[PubMed]
Kang MH et al: "Adenosine-triphosphate-binding cassette transporter-1 trafficking and function."
No.
Sentence
Comment
133
Inhibiting this modification either pharmacologically or by mutating the sites of palmitoylation (C3S/C23S/C1110S/C1111S) (Figure 2) dramatically reduces ABCA1 at the cell surface and decreases cholesterol and phospholipid efflux to apoA-I.
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ABCA1 p.Cys23Ser 20656214:133:102
status: NEW134 Inhibiting this modification either pharmacologically or by mutating the sites of palmitoylation (C3S/C23S/C1110S/C1111S) (Figure 2) dramatically reduces ABCA1 at the cell surface and decreases cholesterol and phospholipid efflux to apoA-I.
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ABCA1 p.Cys23Ser 20656214:134:102
status: NEW
PMID: 19556522
[PubMed]
Singaraja RR et al: "Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function."
No.
Sentence
Comment
100
Circulation Research July 17, 2009 at C23S, and the double C3/C23S.
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ABCA1 p.Cys23Ser 19556522:100:39
status: NEWX
ABCA1 p.Cys23Ser 19556522:100:63
status: NEW129 We generated stable cell lines harboring each of the C3S, C23S, C1110S, and C1111S mutants singly and in combination and determined the extent of both cholesterol and phospholipid efflux to ApoA-I facilitated by these cell lines.
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ABCA1 p.Cys23Ser 19556522:129:58
status: NEW135 C23S .....……………………….……..……...…S………………..……………………………….….
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ABCA1 p.Cys23Ser 19556522:135:0
status: NEW136 C3S/C23S .....S.…………….……….…….…......S…………………..………..…..………………… C1110S/C1111S ....……………………………………………………..………………..…..….SS……….…….
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ABCA1 p.Cys23Ser 19556522:136:4
status: NEW137 C3S/C23S/ C1110S/C1111S .....S.………………………......…......S……………..…..…..….....SS…..………….
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ABCA1 p.Cys23Ser 19556522:137:4
status: NEW138 B C3/C23/C1110/C1111S C3/C23SC 100KDa W T C3S C23S IP: α-His WB: α-Myc 75KDa 100KDa 75KDa 3H fluorogram W T C3S C23S C3/C23S C1110/C1111S WB: α-ABCA1 3H fluorogram 250KDa 250KDa D COOHH2N B A B A C C C3S C23S C1110S C1111S WT C3/C23/C1110/C1111S Palmitoylation (Normalizedtototalprotein) E 0 20 40 60 80 100 120 p=0.000002 W T C3/23/1110/1111SW T C3/23/1110/1111SW T C3/23/1110/1111S 3H fluorogram ABCA1 ABCA1 250KDa 250KDa WB: α-ABCA1 Figure 3.
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ABCA1 p.Cys23Ser 19556522:138:46
status: NEWX
ABCA1 p.Cys23Ser 19556522:138:124
status: NEWX
ABCA1 p.Cys23Ser 19556522:138:132
status: NEWX
ABCA1 p.Cys23Ser 19556522:138:222
status: NEW95 Circulation Research July 17, 2009 C23S, and the double C3/C23S.
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ABCA1 p.Cys23Ser 19556522:95:36
status: NEWX
ABCA1 p.Cys23Ser 19556522:95:60
status: NEW124 We generated stable cell lines harboring each of the C3S, C23S, C1110S, and C1111S mutants singly and in combination and determined the extent of both cholesterol and phospholipid efflux to ApoA-I facilitated by these cell lines.
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ABCA1 p.Cys23Ser 19556522:124:58
status: NEW130 C23S .....ߪߪߪߪߪߪߪߪߪ.ߪߪ..ߪߪ...ߪSߪߪߪߪߪߪ..ߪߪߪߪߪߪߪߪߪߪߪߪ.ߪ.
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ABCA1 p.Cys23Ser 19556522:130:0
status: NEW131 C3S/C23S .....S.ߪߪߪߪߪ.ߪߪߪ.ߪߪ.ߪ......Sߪߪߪߪߪߪߪ..ߪߪߪ..ߪ..ߪߪߪߪߪߪߪ C1110S/C1111S ....ߪߪߪߪߪߪߪߪߪߪߪߪߪߪߪߪߪߪߪߪ..ߪߪߪߪߪߪ..ߪ..ߪ.SSߪߪߪ.ߪߪ.
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ABCA1 p.Cys23Ser 19556522:131:4
status: NEW132 C3S/C23S/ C1110S/C1111S .....S.ߪߪߪߪߪߪߪߪߪ......ߪ......Sߪߪߪߪߪ..ߪ..ߪ..ߪ.....SSߪ..ߪߪߪߪ.
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ABCA1 p.Cys23Ser 19556522:132:4
status: NEW133 B C 3 / C 2 3 / C 1 1 1 0 / C 1 1 1 1 S C3/C23S C 100KDa W T C3S C23S IP: b1;-His WB: b1;-Myc 75KDa 100KDa 75KDa 3H fluorogram W T C 3 S C 2 3 S C 3 / C 2 3 S C 1 1 1 0 / C 1 1 1 1 S WB: b1;-ABCA1 3H fluorogram 250KDa 250KDa D COOH H2N B A B A C C C3S C23S C1110S C1111S WT C3/C23/C1110/C1111S Palmitoylation (Normalized to total protein) E 0 20 40 60 80 100 120 p=0.000002 W T C 3 / 2 3 / 1 1 1 0 / 1 1 1 1 S W T C 3 / 2 3 / 1 1 1 0 / 1 1 1 1 S W T C 3 / 2 3 / 1 1 1 0 / 1 1 1 1 S 3H fluorogram ABCA1 ABCA1 250KDa 250KDa WB: b1;-ABCA1 Figure 3.
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ABCA1 p.Cys23Ser 19556522:133:43
status: NEWX
ABCA1 p.Cys23Ser 19556522:133:65
status: NEWX
ABCA1 p.Cys23Ser 19556522:133:261
status: NEW