ABCMdb

ABCA1 p.Cys1111Ser

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Publications

PMID: 20656214 [PubMed] Kang MH et al: "Adenosine-triphosphate-binding cassette transporter-1 trafficking and function."

No. Sentence Comment

133 Inhibiting this modification either pharmacologically or by mutating the sites of palmitoylation (C3S/C23S/C1110S/C1111S) (Figure 2) dramatically reduces ABCA1 at the cell surface and decreases cholesterol and phospholipid efflux to apoA-I. Login to comment
134 Inhibiting this modification either pharmacologically or by mutating the sites of palmitoylation (C3S/C23S/C1110S/C1111S) (Figure 2) dramatically reduces ABCA1 at the cell surface and decreases cholesterol and phospholipid efflux to apoA-I. Login to comment

PMID: 19556522 [PubMed] Singaraja RR et al: "Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function."

No. Sentence Comment

108 Assessment of palmitoylation of these sites in the context of full-length ABCA1 showed that ABCA1 was still palmitoylated (Figure 3D, lane C1110/C1111S). Login to comment
109 However, generation of the quadruple mutant C3/23/1110/1111S in full-length ABCA1 resulted in a significant reduction in ABCA1 palmitoylation (Figure 3D, lane C3/C23/C1110/C1111S; and Figure 3E). Login to comment
129 We generated stable cell lines harboring each of the C3S, C23S, C1110S, and C1111S mutants singly and in combination and determined the extent of both cholesterol and phospholipid efflux to ApoA-I facilitated by these cell lines. Login to comment
136 C3S/C23S .....S.…………….……….…….…......S…………………..………..…..………………… C1110S/C1111S ....……………………………………………………..………………..…..….SS……….……. Login to comment
137 C3S/C23S/ C1110S/C1111S .....S.………………………......…......S……………..…..…..….....SS…..…………. Login to comment
138 B C3/C23/C1110/C1111S C3/C23SC 100KDa W T C3S C23S IP: α-His WB: α-Myc 75KDa 100KDa 75KDa 3H fluorogram W T C3S C23S C3/C23S C1110/C1111S WB: α-ABCA1 3H fluorogram 250KDa 250KDa D COOHH2N B A B A C C C3S C23S C1110S C1111S WT C3/C23/C1110/C1111S Palmitoylation (Normalizedtototalprotein) E 0 20 40 60 80 100 120 p=0.000002 W T C3/23/1110/1111SW T C3/23/1110/1111SW T C3/23/1110/1111S 3H fluorogram ABCA1 ABCA1 250KDa 250KDa WB: α-ABCA1 Figure 3. Login to comment
169 A D p=0.0007 p=0.04 p=0.002 p=0.0005 p=0.0002 p<0.0001 0 20 40 60 80 100 120 W T C 3S C 23SC 3S/C 23S C 1110S/C 1111S C 3/C 23/C 1110/C 1111S M 1091T %cholesterolefflux(normalizedtowt) p=0.003 p=0.009p=0.01 p=0.03 p=0.03 p<0.0001 0 20 40 60 80 100 120 W T C 3S C 23SC 3S/C 23S C 1110S/C 1111S C 3/C 23/C 1110/C 1111S M 1091T %phosphocholineefflux(normalizedtowt) Empty Vector WT- No Biotin 150 100 250 75 50 37 Input IP Input IP Input IP WT+Biotin C3/C23/ C1110/C111S+Biotin ABCA1 GAPDH Controls WB: αABCA1 WB: αGAPDH C3/C23/C1110/C1111S 0 20 40 60 80 100 120 p=0.00003 W T BiotinlabeledABCA1(normalizedtoWT) Input IP B C Figure 5. Login to comment
103 Assessment of palmitoylation of these sites in the context of full-length ABCA1 showed that ABCA1 was still palmitoylated (Figure 3D, lane C1110/C1111S). Login to comment
104 However, generation of the quadruple mutant C3/23/1110/1111S in full-length ABCA1 resulted in a significant reduction in ABCA1 palmitoylation (Figure 3D, lane C3/C23/C1110/C1111S; and Figure 3E). Login to comment
124 We generated stable cell lines harboring each of the C3S, C23S, C1110S, and C1111S mutants singly and in combination and determined the extent of both cholesterol and phospholipid efflux to ApoA-I facilitated by these cell lines. Login to comment
131 C3S/C23S .....S.ߪߪߪߪߪ.ߪߪߪ.ߪߪ.ߪ......Sߪߪߪߪߪߪߪ..ߪߪߪ..ߪ..ߪߪߪߪߪߪߪ C1110S/C1111S ....ߪߪߪߪߪߪߪߪߪߪߪߪߪߪߪߪߪߪߪߪ..ߪߪߪߪߪߪ..ߪ..ߪ.SSߪߪߪ.ߪߪ. Login to comment
132 C3S/C23S/ C1110S/C1111S .....S.ߪߪߪߪߪߪߪߪߪ......ߪ......Sߪߪߪߪߪ..ߪ..ߪ..ߪ.....SSߪ..ߪߪߪߪ. Login to comment
133 B C 3 / C 2 3 / C 1 1 1 0 / C 1 1 1 1 S C3/C23S C 100KDa W T C3S C23S IP: b1;-His WB: b1;-Myc 75KDa 100KDa 75KDa 3H fluorogram W T C 3 S C 2 3 S C 3 / C 2 3 S C 1 1 1 0 / C 1 1 1 1 S WB: b1;-ABCA1 3H fluorogram 250KDa 250KDa D COOH H2N B A B A C C C3S C23S C1110S C1111S WT C3/C23/C1110/C1111S Palmitoylation (Normalized to total protein) E 0 20 40 60 80 100 120 p=0.000002 W T C 3 / 2 3 / 1 1 1 0 / 1 1 1 1 S W T C 3 / 2 3 / 1 1 1 0 / 1 1 1 1 S W T C 3 / 2 3 / 1 1 1 0 / 1 1 1 1 S 3H fluorogram ABCA1 ABCA1 250KDa 250KDa WB: b1;-ABCA1 Figure 3. Login to comment
164 A D p=0.0007 p=0.04 p=0.002 p=0.0005 p=0.0002 p<0.0001 0 20 40 60 80 100 120 W T C 3 S C 2 3 S C 3 S / C 2 3 S C 1 1 1 0 S / C 1 1 1 1 S C 3 / C 2 3 / C 1 1 1 0 / C 1 1 1 1 S M 1 0 9 1 T % cholesterol efflux (normalized to wt) p=0.003 p=0.009p=0.01 p=0.03 p=0.03 p<0.0001 0 20 40 60 80 100 120 W T C 3 S C 2 3 S C 3 S / C 2 3 S C 1 1 1 0 S / C 1 1 1 1 S C 3 / C 2 3 / C 1 1 1 0 / C 1 1 1 1 S M 1 0 9 1 T %phosphocholine efflux (normalized to wt) Empty Vector WT- No Biotin 150 100 250 75 50 37 Input IP Input IP Input IP WT+Biotin C3/C23/ C1110/C111S+Biotin ABCA1 GAPDH Controls WB: b1;ABCA1 WB: b1;GAPDH C3/C23/C1110/C1111S 0 20 40 60 80 100 120 p=0.00003 W T Biotin labeled ABCA1 (normalized to WT) Input IP B C Figure 5. Login to comment