ABCC8 p.Asp96Glu
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PMID: 10908566
[PubMed]
Garinot-Schneider C et al: "Identification of essential amino acid residues in the Sinorhizobium meliloti glucosyltransferase ExoM."
No.
Sentence
Comment
131
However, the two mutants N45A and D96E were not detected on Coomassie Blue-stained SDS-PAGE gels (Fig. 3).
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ABCC8 p.Asp96Glu 10908566:131:34
status: NEW144 Unfortunately, the mutant protein containing the conservative D96E mutation was not stably expressed in E. coli (Fig. 3) but, as will be presented below, was stably expressed in S. meliloti.
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ABCC8 p.Asp96Glu 10908566:144:62
status: NEW163 However, of the conservative mutations D44E, D96E, and D187E, only D187E was completely incapable of restoring succinoglycan production.
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ABCC8 p.Asp96Glu 10908566:163:45
status: NEW164 S. meliloti, harboring ExoM D44E and ExoM D96E, was able to produce 77 and 41% of the wild type succinoglycan level, respectively, demonstrating that these conservative mutations do not completely inactivate the protein.
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ABCC8 p.Asp96Glu 10908566:164:42
status: NEW175 In vitro activity Succinoglycan production (%wt) %wt ExoM 100 100 Site I V42I 100 69 A43D 3 Ͼ3 D44Aa 0 Ͼ3 D44E 7 77 N45A NEb NE D46A 75 NE Site II F94V 69 77 L95A 30 114 D96A 0 7 D96E NE 41 D97A 15 115 D98A 26 139 E99A 100 84 Site III D187A 0 3 D187E 0 4 a The mutations involving conserved amino acid residues are in italics. b NE, not expressed.
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ABCC8 p.Asp96Glu 10908566:175:191
status: NEW