ABCC8 p.Ser1448Ala
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PMID: 12198249
[PubMed]
Light PE et al: "Glucagon-like peptide-1 inhibits pancreatic ATP-sensitive potassium channels via a protein kinase A- and ADP-dependent mechanism."
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Sentence
Comment
8
The point mutation S1448A in the ADP-sensing region of SUR1 removed the modulatory effects of cPKA.
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ABCC8 p.Ser1448Ala 12198249:8:19
status: NEW87 We therefore generated a S1448A substitution mutant in the hamster SUR1 sequence and coexpressed this mutant with Kir6.2.
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ABCC8 p.Ser1448Ala 12198249:87:25
status: NEW88 Inside-out patch experiments revealed that the S1448A mutant was functionally expressed and possessed similar ATP and ADP-sensing properties when compared with WT SUR1/Kir6.2 channels (Fig. 5D).
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ABCC8 p.Ser1448Ala 12198249:88:47
status: NEW89 Under the identical conditions that produced a cPKA- induced significant reduction in WT KATP channel current (0.2 mM ATP and 0.2 mM ADP), SUR1(S1448A)/ Kir6.2 current was not significantly changed in the presence of 20 nM cPKA [95.40 Ϯ 2.8%, n ϭ 8 patches (P Ͼ 0.05), Fig. 5, A and C].
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ABCC8 p.Ser1448Ala 12198249:89:144
status: NEW99 cPKA, the SUR1(S1448A)/Kir6.2 current was also unaffected by application of cPKA (95.31 Ϯ 5.94, n ϭ 13 patches, Fig. 5B).
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ABCC8 p.Ser1448Ala 12198249:99:15
status: NEW146 The Effects of Purified PKA on Recombinant WT or KATP Channels Expressing the SUR1 Substitution Mutant, SUR1(S1448A) A and B, Representative recordings of Kir6.2/SUR1(S1448A) currents from excised inside-out membrane patches from tsA201 cells in response to application of 20 nM cPKA in the presence of either 0.2 mM ADP (A) or 0.5 mM ADP (B).
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ABCC8 p.Ser1448Ala 12198249:146:109
status: NEWX
ABCC8 p.Ser1448Ala 12198249:146:167
status: NEW148 C, Grouped data from WT (Kir6.2/ SUR1) or mutated (S1448A) KATP channels in response to application of cPKA, either in the presence or absence of ADP (0.2 mM) and ATP (0.2 mM) ATP continuously present.
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ABCC8 p.Ser1448Ala 12198249:148:51
status: NEW150 D, Grouped data, from inside-out patch experiments with WT and S1448A mutant KATP channels, showing a similar response to ATP alone (0.2 mM) and a release of this ATP-induced inhibition by ADP (0.2 mM).
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ABCC8 p.Ser1448Ala 12198249:150:63
status: NEW151 There were no statistical differences in nucleotide sensitivities between WT and S1448A KATP channels.
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ABCC8 p.Ser1448Ala 12198249:151:81
status: NEW196 Molecular Biology The S1448A point mutation was introduced into the hamster SUR1 clone, using the Unique Site Elimination (U.S.E.) Mutagenesis Kit as per the manufacturer`s instructions (Amersham Pharmacia Biotech, Piscataway, NJ) and confirmed by sequence analysis.
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ABCC8 p.Ser1448Ala 12198249:196:22
status: NEW