ABCB6 p.Cys120Ala
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PMID: 21199866
[PubMed]
Fukuda Y et al: "Conserved intramolecular disulfide bond is critical to trafficking and fate of ATP-binding cassette (ABC) transporters ABCB6 and sulfonylurea receptor 1 (SUR1)/ABCC8."
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61
The region encoding the amino-terminal 210 amino acids was then amplified with a FLAG tag at the carboxyl terminus from pcDNA3.1-hABCB6-C50A/C120A-V5 or pcDNA3.1-hABCB6-C8S/C50A/C120A-V5 by using primers (sense, 5Ј-GCCATGGTGACTGTGGGCAACTACTG- CGAGGCCG-3Ј; antisense, 5Ј-CCTACTTATCGTCGTCATC- CTTGTAATCACGAAGTCCAGGGGCCCAGAG-3Ј) under the following conditions: heating at 94 °C for 4 min, 30 cycles of denaturation at 94 °C for 30 s, annealing at 56 °C for 30 s, and reaction at 68 °C for 1 min.
X
ABCB6 p.Cys120Ala 21199866:61:141
status: NEWX
ABCB6 p.Cys120Ala 21199866:61:178
status: NEW