ABCC8 p.Arg74Asp
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PMID: 21321069
[PubMed]
Pratt EB et al: "N-terminal transmembrane domain of SUR1 controls gating of Kir6.2 by modulating channel sensitivity to PIP2."
No.
Sentence
Comment
100
Because of significantly reduced surface expression in most mutants, cells were pretreated with 300 µM tolbutamide overnight, which partially corrects the trafficking defect caused by R74W, as reported previously (Yan et al., 2004, 2007; Pratt et al., 2009), as well as all other R74 mutations, with the exception of R74D (Fig. S1).
X
ABCC8 p.Arg74Asp 21321069:100:321
status: NEW101 Because R74D had no detectable surface expression before or after tolbutamide exposure, it was not analyzed.
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ABCC8 p.Arg74Asp 21321069:101:8
status: NEW263 Accordingly, with the exception of lysine, all other amino acids used in the R74X screen (A, H, C, W, F, Y, L, D, or E) are predicted to shift the boundary of TM1 and TM2 (as well as TM3 for R74D and R74E) by TOPCONS (Fig. S2).
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ABCC8 p.Arg74Asp 21321069:263:191
status: NEW265 Sulfonylureas acting as chemical chaperones partially overcome the trafficking defect in R74X full-length KATP channels, with the exception of R74D (Fig. S1).
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ABCC8 p.Arg74Asp 21321069:265:143
status: NEW