ABCC8 p.Ser1482Arg
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No.
Sentence
Comment
184
Mutations within the ABC signature motif of NBF2 of SUR1 (S1482R) and SUR2B (S1446R) abolished the channel activation by MgADP, whereas the corresponding mutation of NBF1 of SUR1 (S830R) and SUR2B (S809R) did not [96].
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ABCC8 p.Ser1482Arg 15910875:184:58
status: NEW
PMID: 12169627
[PubMed]
Matsuo M et al: "Mutations in the linker domain of NBD2 of SUR inhibit transduction but not nucleotide binding."
No.
Sentence
Comment
149
Table I. Percentage of photoaf®nity labelling by 8-azido-[a-32P]ATP remaining after displacement with unlabelled ATP or ADP NBD1 (%) NBD2 (%) ATP ADP ATP ADP SUR1 12 T 10 21 T 14 52 T 11 45 T 11 SUR1-S830R 8 T 3.7 25 T 9.0 43 T 11 33 T 6.8 SUR1-S1482R 9 T 5.1 21 T 11 39 T 14 34 T 8.4 Values are expressed as a percentage of 8-azido-[a-32P]ATP bound in the absence of unlabelled ATP (100 mM) or ADP (100 mM).
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ABCC8 p.Ser1482Arg 12169627:149:249
status: NEW239 Mutations in the NBD2 linker were: SUR1-S1482R and SUR2-S1446R (S2R); SUR1-Q1426A, SUR1-H1537A.
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ABCC8 p.Ser1482Arg 12169627:239:40
status: NEW260 Crude membranes, containing similar amounts of wild-type SUR1, SUR1-S830R and SUR1-S1482R, as determined by western blotting, were incubated with 50 mM 8-azido- [a-32P]ATP or 50 mM 8-azido-[g-32P]ATP (ICN Biomedicals) in the presence or absence of 100 mM ATP or 100 mM ADP in 3 ml of TEM buffer (40 mM Tris±HCl pH 7.5, 0.1 mM EGTA and 1 mM MgSO4) containing 2 mM ouabain.
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ABCC8 p.Ser1482Arg 12169627:260:83
status: NEW