ABCMdb

ABCC8 p.Leu183Ala

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Publications

PMID: 21685390 [PubMed] Marquer C et al: "Structural model of ligand-G protein-coupled receptor (GPCR) complex based on experimental double mutant cycle data: MT7 snake toxin bound to dimeric hM1 muscarinic receptor."

No. Sentence Comment

156 hM1 mutant Region pKi NMS pKX MT7 KX(mut)/KX(WT) WT 10.22 Ϯ 0.04 10.47 Ϯ 0.04 H90A E1 10.20 Ϯ 0.09 10.66 Ϯ 0.20 0.6 W91A E1 9.23 ؎ 0.33 9.30 ؎ 0.06 14.9 W101A TM3 9.29 Ϯ 0.02 9.79 Ϯ 0.13 4.8 Y166A E2 9.83 Ϯ 0.03 10.33 Ϯ 0.32 1.4 L167F E2 9.93 Ϯ 0.02 10.48 Ϯ 0.11 1.0 E170A E2 10.20 Ϯ 0.04 10.20 Ϯ 0.07 1.9 E170K E2 10.16 Ϯ 0.02 9.31 ؎ 0.11 14.6 R171A E2 9.76 Ϯ 0.01 9.50 ؎ 0.29 9.4 L174A E2 10.21 Ϯ 0.08 10.51 Ϯ 0.01 0.9 L174P E2 10.15 Ϯ 0.01 9.37 ؎ 0.14 12.5 Q177E E2 10.08 Ϯ 0.04 10.58 Ϯ 0.17 0.8 Y179F E2 9.85 Ϯ 0.02 9.25 ؎ 0.18 16.5 L183A E2 10.00 Ϯ 0.11 10.44 Ϯ 0.20 1.1 Q185A E2 10.18 Ϯ 0.10 10.59 Ϯ 0.37 0.8 K392A E3 10.20 Ϯ 0.12 10.57 Ϯ 0.18 0.8 D393A E3 9.96 Ϯ 0.09 10.50 Ϯ 0.23 0.9 E397A E3 10.21 Ϯ 0.06 10.54 Ϯ 0.20 0.9 W400A TM7 10.12 Ϯ 0.04 9.43 ؎ 0.02 11.0 E401A TM7 9.76 Ϯ 0.02 10.20 Ϯ 0.10 1.9 TABLE 2 Affinity constants and variations in free energy of interaction of wild-type and modified MT7 toxins for wild-type and mutated hM1 receptors Data were inferred from binding experiments with ͓3 H͔NMS and calculated with the ternary complex equation. Login to comment