ABCB1 p.Phe336Cys
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PMID: 9261097
[PubMed]
Loo TW et al: "Drug-stimulated ATPase activity of human P-glycoprotein requires movement between transmembrane segments 6 and 12."
No.
Sentence
Comment
68
To test these predictions, we introduced pairs of cysteines into a Cys-less mutant of P-glycoprotein to create the mutants F336C/S979C, L339C/V982C, F343C/M986C, G346C/G989C, and P350C/S993C.
X
ABCB1 p.Phe336Cys 9261097:68:123
status: NEW78 No cross-linked product was observed for mutants F336C/S979C and L339C/V982C.
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ABCB1 p.Phe336Cys 9261097:78:49
status: NEW107 Mutants S979C/F336C or L339C/V982C did not yield any cross-linked product even in the presence of ATP or drug substrates (data not shown).
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ABCB1 p.Phe336Cys 9261097:107:14
status: NEW124 Cross-linking was not observed between F336C/S979C or L339C/V982C, even in the presence of ATP or drug substrates FIG. 2.
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ABCB1 p.Phe336Cys 9261097:124:39
status: NEW
No.
Sentence
Comment
211
In contrast, two other potential pairs that lie between the first and second of the four cross-linked pairs within TMs 6 and 12 (Loo & Clarke, 1997), namely F336C/S979C and L339C/V982C, failed to form cross-links.
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ABCB1 p.Phe336Cys 9841738:211:157
status: NEW