ABCB1 p.Ala57Arg
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PMID: 18596043
[PubMed]
Loo TW et al: "Arginines in the first transmembrane segment promote maturation of a P-glycoprotein processing mutant by hydrogen bond interactions with tyrosines in transmembrane segment 11."
No.
Sentence
Comment
151
By contrast, 12 of the mutants (V53R, G54R, T55R, L56R, A57R, A58R, I59R, I60R, G62R, A63R, P66R, and F72R) showed little or no rescue in the presence of drug substrates.
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ABCB1 p.Ala57Arg 18596043:151:56
status: NEW165 TABLE 1 Effects of drug substrates on the maturation of TM1 arginine mutants containing the G251V mutation Mutation (G251V ؉) No drug Cyclosporin A Verapamil Vinblastine Rhodamine None - 111a,c 111 111 11 V52R -a,b 111 111 111 11 V53R 2 - - - - G64R 2 - - - - T55R 2 - - - - L56R 2 - - - - A57R 2 - - - - A58R 2 - - - - I59R 2 - - - - I60R 2 - - - - H61R - 111 1 1 1 G62R 2 - - - - A63R 2 - - - - G64R 1 111 1 1 11 L65R 11 111 11 11 11 P66R 2 1 - - - L67R - 111 111 111 11 M68R 111 111 111 111 111 M69R - 111 - 11 111 L70R - 111 111 111 11 V71R 1 111 111 111 11 F72R 2 1 1 1 1 a Change in the amount of mature (170 kDa) protein in the presence of drug substrate relative to that in the absence of drug substrate.
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ABCB1 p.Ala57Arg 18596043:165:296
status: NEW
PMID: 9287132
[PubMed]
Taguchi Y et al: "Amino acid substitutions in the first transmembrane domain (TM1) of P-glycoprotein that alter substrate specificity."
No.
Sentence
Comment
72
We also failed to detect the mature form of P-glycoprotein, when Ala57 -> Arg, Ala58 -> Arg, Ile59 ->Arg, and Gly62 -> Arg mutant cDNAs were transiently expressed in HEK 293 cells (data not shown).
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ABCB1 p.Ala57Arg 9287132:72:65
status: NEW86 However, from cells transfected with Ala57 -> Arg, Ala58 -> Arg, He59 -> Arg, or Gly62 -> Arg mutant cDNA, no Vbl- or Col-resistant colonies were obtained (Fig. 1).
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ABCB1 p.Ala57Arg 9287132:86:37
status: NEW